A novel genetic variant potentially altering the expression of in the cerebellum associated with attention deficit hyperactivity disorder in Han Chinese children.

OBJECTIVES

To obtain additional insight into the genetic factors of attention deficit hyperactivity disorder (ADHD).

METHODS

First, we performed a transcriptome-wide association study (TWAS) integrating human cerebellum-specific variant-expression/splicing correlations to identify ADHD susceptibility genes. Then, the associations between expression/splicing quantitative trait loci (eQTLs/sQTLs) of the transcriptome-wide significant genes and ADHD were observed in a case-control study of Han Chinese children. Furthermore, dual luciferase reporter gene assays were performed to validate the regulatory function of ADHD risk variants. Additionally, the transcription level of target genes in blood was detected by real-time quantitative polymerase chain reaction (RT-qPCR) assay.

RESULTS

TWAS identified that the genetically regulated expression of in the cerebellum was significantly associated with ADHD risk. Furthermore, we observed a higher risk of ADHD and more severe clinical symptoms in subjects harbouring heterozygous (TC) or mutant homozygous (TT) genotypes of rs1054037 than CC carriers. The dual luciferase reporter gene assay revealed that the mutation of rs1054037(C > T) potentially upregulated expression by eliminating the binding site for hsa-miR-5591-3P. Finally, RT-qPCR showed that expression in blood samples of patients was significantly higher than that of controls.

CONCLUSIONS

Taken together, these results suggest a role of in the development of ADHD.