sp. nov., a madurastatin A1-producing strain isolated from lichen in Thailand.

An actinomycete strain, LCR2-06, isolated from a lichen sample on rock collected from Chiang Rai Province (Pong Phra Bat Waterfall), Thailand, was characterized using a polyphasic approach. The strain grew at 25-45 °C, pH 6-11 and on International Project 2 agar plate with 5 % (w/v) NaCl. It contained -diaminopimelic acid as the diamino acid in whole-cell hydrolysates. Rhamnose, ribose, xylose, madurose, glucose and galactose were detected as whole-cell sugar hydrolysates. Mycolic acids were absent. The -acyl type of muramic acid was acetyl. The strain contained C, TBSA 10-methyl C and 2-hydroxy C as the predominant fatty acids and MK-9(H), MK-9(H) and MK-9(H) as the major menaquinones. The major polar lipids were diphosphatidylglycerol, phosphatidylinositol and unidentified phospholipid. The draft genome of strain LCR2-06 was closely related to TBRC 7225 (99.2 %), NBRC 15918 (98.8 %), TISTR 2400 (98.5 %) and JCM 33455 (97.9 %). The draft genome of LCR2-06 was 11.1 Mb with 10 588 coding sequences with an average G+C content of 72.7 mol%. Results of genomic analysis revealed that the ANIb and ANIm values between strain LCR2-06 and TISTR 2400 were 90.0 and 92.0 %, respectively. The digital DNA-DNA hybridization value was 43.9 % in comparison with the draft genome of TISTR 2400. The strain produced an antibacterial compound active against ATCC 6633 and ATCC 9341. The results of taxonomic analysis suggested that strain LCR2-06 represented a novel species of the genus for which the name sp. nov. is proposed. The type strain is LCR2-06 (=JCM 33065=KCTC 49547=NBRC 114810=LMG 32136=TISTR 2935).