sp. nov., isolated from the foliose lichen, (Nyl.) Hale.

A novel actinomycete strain PM05-2 was isolated from the lichen (Nyl.) Hale collected from Chaiyaphum Province, Thailand. The taxonomic position of the strain was studied using the polyphasic approach. Based on the morphology and chemotaxonomic properties, strain PM05-2 was identified as a member of the genus . The whole-cell hydrolysate contained -diaminopimelic acid, rhamnose, ribose, xylose, madurose, glucose and galactose. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified phosphoglycolipid, four unidentified phospholipids and one unidentified lipid. The menaquinones were MK-9(H), MK-9(H), MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids were C and C ω9. Strain PM05-2 showed the highest 16S rRNA gene similarity to NBRC 15177 (98.58%), NBRC 14229 (98.29 %) and DSM 44197 (98.14 %). The phylogenetic tree analysis revealed that strain PM05-2 was related to NBRC 15177, NBRC 14229, DSM 44197 and RB68. The genomic analysis revealed that average nucleotide identity values based on both blast and MUMmer between strain PM05-2 and the relative type strains ranged from 77.6 to 86.4%. The digital DNA-DNA hybridization values among the strains were lower than the threshold for assigning to the same species. The taxonomic results suggested that strain PM05-2 represented a novel species of the genus for which the name is proposed. The type strain is PM05-2 (=TBRC 15492=NBRC 115416).