Department of Cellular, Computational and Integrative Biology - CIBIO, University of Trento, 38123 Trento, Italy.
Faculty of Sciences and Technology, Libera Università di Bolzano, 39100 Bolzano, Italy.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005131.
Strain 3P27G6 was isolated from an artesian well connected to the thermal water basin of Comano Terme, Province of Trento, Italy. In phylogenetic analyses based on multilocus sequence analysis, strain 3P27G6 clustered together with WSM2073. Genome sequencing produced a 99.51 % complete genome, with a length of 7 363 057 bp and G+C content of 63.53 mol%, containing 6897 coding sequences, 55 tRNA and three rRNA. Average nucleotide identity analysis revealed that all distances calculated between strain 3P27G6 and the other genomes were below 0.9, indicating that strain 3P27G6 represents a new species. Therefore, we propose the name sp. nov. with the type strain 3P27G6 (=DSM 110654=CECT 30067). Strain 3P27G6 is a Gram-negative, rod-shaped, aerobic bacterium. Growth condition, antibiotic susceptibility, metabolic and fatty acid-methyl esters profiles of the strain were determined. Only few nodulation and nitrogen fixation genes were found in the genome, suggesting that this strain may not be specialized in nodulation or in nitrogen fixation.
菌株 3P27G6 是从意大利特伦托省卡莫诺温泉区的自流井中分离出来的。基于多位点序列分析的系统发育分析表明,菌株 3P27G6 与 WSM2073 聚在一起。基因组测序产生了一个 99.51%完整的基因组,长度为 7363057bp,GC 含量为 63.53mol%,包含 6897 个编码序列、55 个 tRNA 和 3 个 rRNA。平均核苷酸同一性分析表明,菌株 3P27G6 与其他基因组之间计算的所有距离均低于 0.9,表明菌株 3P27G6 代表一个新物种。因此,我们建议使用 3P27G6 株(=DSM 110654=CECT 30067)作为模式株,命名为 sp. nov.。菌株 3P27G6 是革兰氏阴性、杆状、需氧细菌。确定了该菌株的生长条件、抗生素敏感性、代谢和脂肪酸甲酯图谱。基因组中只发现了少数固氮和结瘤基因,这表明该菌株可能不专门用于固氮或结瘤。