Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
National Institute of Biological Resources, Incheon 22689, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004847.
Strain MaA-C15, a Gram-stain-negative, non-spore-forming and strictly aerobic bacterium, was isolated from a xenic culture of in the Republic of Korea. Cells were motile rods showing positive reactions in catalase and oxidase tests. Growth was observed between 15 and 37 °C (optimum, 30 °C), between pH 6.0 and pH 11.0 (optimum, pH 7.5) and in the presence of 0-2.0 % (w/v) NaCl (optimum, 0 %). Strain MaA-C15 contained C, 11-methyl-C 7, cyclo-C 8 and summed feature 8 (C 6 and/or C 7) as the major cellular fatty acids and ubiquinone-10 as the sole respiratory quinone. Phosphatidylethanolamine, phosphatidylmonomethylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid and three unidentified phospholipids were detected as the major polar lipids. The G+C content of the genomic DNA was 64.1 mol%. Phylogenetic and phylogenomic analyses based on 16S rRNA gene and genome sequences revealed that strain MaA-C15 formed a phyletic lineage with YIM M12096 within the family . Strain MaA-C15 was most closely related to DSM 21822 with a 98.2 % 16S rRNA sequence similarity. Average nucleotide identity and DNA-DNA hybridization values between strain MaA-C15 and DSM 21822 were 75.4 and 20.1 %, respectively. Based on the results of phenotypic, chemotaxonomic and molecular analyses, strain MaA-C15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MaA-C15 (=KACC 21226=JCM 33503).
马氏菌 MaA-C15 株，革兰氏阴性、非孢子形成且严格需氧的细菌，从韩国共生培养物中分离得到。细胞为运动杆状，过氧化氢酶和氧化酶试验呈阳性反应。在 15-37°C（最佳温度 30°C）、pH6.0-11.0（最佳 pH7.5）和 0-2.0%（w/v）NaCl（最佳 0%）条件下生长。菌株 MaA-C15 含有 C11-methyl-C7、cyclo-C8 和总和特征 8（C6 和/或 C7）作为主要的细胞脂肪酸，以及泛醌-10 作为唯一的呼吸醌。鉴定出磷脂酰乙醇胺、磷脂单甲基乙醇胺、一种未鉴定的氨基磷脂、一种未鉴定的糖脂和三种未鉴定的磷脂作为主要的极性脂。基因组 DNA 的 G+C 含量为 64.1mol%。基于 16S rRNA 基因和基因组序列的系统发育和系统基因组分析表明，菌株 MaA-C15 与家族 内的 YIM M12096 形成了一个分支。菌株 MaA-C15 与 DSM 21822 的亲缘关系最近，16S rRNA 序列相似性为 98.2%。菌株 MaA-C15 与 DSM 21822 的平均核苷酸同一性和 DNA-DNA 杂交值分别为 75.4%和 20.1%。基于表型、化学分类和分子分析的结果，菌株 MaA-C15 代表了一个新的属种，提议命名为. 模式菌株为 MaA-C15（=KACC 21226=JCM 33503）。
