Microbe Division/Japan Collection of Microorganisms, RIKEN BioResource Research Center, Tsukuba, Ibaraki 305-0074, Japan.
Advanced Genomics Center, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005121.
Two obligately anaerobic, Gram-stain-positive, rod-shaped bacteria were isolated from faecal samples of healthy humans in Japan. 16S rRNA gene sequence analysis indicated that these two strains (8CFCBH1 and 9CBH6) belonged to the genus , which is known as an equol-producing bacterium. The closest neighbours of strain 8CFCBH1 were subsp. DSM 19450 (98.6%), subsp. do03 (98.4%), WCA-131-CoC-2 (96.6%), NR06 (96.4%), B7 (95.3%) and Mt1B8 (95.3%). The closest relatives to strain 9CBH6 were subsp. DSM 19450 (99.8%), subsp. do03 (99.6%) and WCA-131-CoC-2 (96.8%). Strain 8CFCBH1 showed 22.3-53.5% digital DNA-DNA hybridization (dDDH) values with its related species. In addition, the average nucleotide identity (ANI) values between strain 8CFCBH1 and its related species ranged from 75.4 to 93.3%. On the other hand, strain 9CBH6 was considered as based on the dDDH and ANI values (>70% dDDH and >95-96% ANI). Strain 9CBH6 showed daidzein-converting activity, as expected from the result of genome analysis. The genome of strain 8CFCBH1 lacked four genes involved in equol production. Growing cells of strain 8CFCBH1 were not capable of converting daidzein. Based on the collected data, strain 8CFCBH1 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is 8CFCBH1 (=JCM 34083=DSM 112284).
从日本健康人群的粪便样本中分离到两株严格厌氧、革兰氏阳性、杆状细菌。16S rRNA 基因序列分析表明，这两株菌（8CFCBH1 和 9CBH6）属于 属，该属已知是一种能够产生雌马酚的细菌。菌株 8CFCBH1 的最接近的近缘种是 subsp. DSM 19450（98.6%）、 subsp. do03（98.4%）、WCA-131-CoC-2（96.6%）、NR06（96.4%）、B7（95.3%）和 Mt1B8（95.3%）。菌株 9CBH6 的最接近的亲缘种是 subsp. DSM 19450（99.8%）、 subsp. do03（99.6%）和 WCA-131-CoC-2（96.8%）。菌株 8CFCBH1 与其相关种之间的数字 DNA-DNA 杂交（dDDH）值为 22.3-53.5%。此外，菌株 8CFCBH1 与其相关种之间的平均核苷酸同一性（ANI）值在 75.4%至 93.3%之间。另一方面，根据 dDDH 和 ANI 值，菌株 9CBH6 被认为是 （dDDH 大于 70%，ANI 大于 95-96%）。菌株 9CBH6 表现出大豆黄素转化活性，这与基因组分析的结果一致。菌株 8CFCBH1 的基因组缺乏参与雌马酚生成的四个基因。生长中的菌株 8CFCBH1 不能将大豆黄素转化。基于收集到的数据，菌株 8CFCBH1 代表了 属的一个新种，建议将其命名为 sp. nov.。的模式菌株是 8CFCBH1（=JCM 34083=DSM 112284）。
