一步提取同时定量荧光免疫层析试纸条用于粮食中 AFB 和 Cd 的检测。
One-step extraction and simultaneous quantitative fluorescence immunochromatography strip for AFB and Cd detection in grain.
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Food, Synergetic Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, China.
Academy of National Food and Strategic Reserves Administration, No.11 Baiwanzhuang Str, Xicheng District, Beijing 100037, China.
出版信息
Food Chem. 2022 Apr 16;374:131684. doi: 10.1016/j.foodchem.2021.131684. Epub 2021 Dec 1.
AFB and heavy metal Cd are two common pollutants during grain storage. The rapid detection of grains before they enter the granary is particularly important. Hence, rapidly, accurately, and sensitively screening contaminated grains, simplifying the detection process, and reducing detection costs are necessary. In this study, linear ranges of time-resolved fluorescence microsphere - immunochromatographic test strip (TRFM-ICTS) detection were 0.01-30 ng/mL (AFB) and 0.01-60 ng/mL (Cd), and the IC values were 0.536 ng/mL (AFB) and 3.331 ng/mL (Cd). In the TRFM-ICTS sample addition experiment, the recovery rates were all between 90% and 110%. The coefficient of variation was less than 8% in the actual sample detection process of grain. We have established a one-step extraction method for AFB and Cd in grains to achieve simultaneous detection in one extraction. In addition, TRFM-ICTS could be stored for at least 12 months, providing technical support for the realization of commercial production.
AFB 和重金属 Cd 是粮食储存过程中的两种常见污染物。在粮食进入粮仓之前快速检测尤为重要。因此，快速、准确、灵敏地筛选污染粮食，简化检测过程，降低检测成本是必要的。本研究中，时间分辨荧光微球免疫层析试纸条（TRFM-ICTS）检测的线性范围分别为 0.01-30ng/mL（AFB）和 0.01-60ng/mL（Cd），IC 值分别为 0.536ng/mL（AFB）和 3.331ng/mL（Cd）。在 TRFM-ICTS 样品添加实验中，回收率均在 90%到 110%之间。在实际粮食样品检测过程中，变异系数小于 8%。我们建立了一种一步提取法用于粮食中 AFB 和 Cd 的同时检测，实现了一次提取的同时检测。此外，TRFM-ICTS 可至少保存 12 个月，为实现商业化生产提供了技术支持。
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