Gibson S, Friedman H
J Clin Microbiol. 1978 Mar;7(3):279-81. doi: 10.1128/jcm.7.3.279-281.1978.
A semisolid medium (designated Serratia differentiation medium) containing L-arabinose, ornithine, and selective inhibitor was used to differentiate three clinically encountered Serratia species. The inhibitor, Irgasan DP-300, was incorporated to eliminate false-positive reactions from most remaining Enterobacteriaceae. The suspected Serratia colony was inoculated as a stab into the medium. Serratia marcescens was indicated by a change in color from olive to purple following 18 h of incubation, whereas S. rubidaea (not listed in Bergey's Manual of Determinative Bacteriology) was indicated by a change to bright yellow. S. liquefaciens (described in Bergey's Manual of Determinative Bacteriology [8th ed., 1974] as Enterobacter liquefaciens) produced a small purple band at the top of the medium and a yellow or yellow-green butt. Absence of growth and color change following incubation indicates that the suspected colony is a non-Serratia. Thirty-six Serratia strains and 97 other Enterobacteriaceae and Pseudomonadaceae strains were tested. Two strains of the non-Serratia Enterobacteriaceae (one each of Citrobacter freundii and Proteus morganii) and two strains of Pseudomonas aeruginosa produced a color change in the medium. All of the Serratia strains tested were correctly identified using this medium, while 96% of the other species tested were inhibited.
一种含有L-阿拉伯糖、鸟氨酸和选择性抑制剂的半固体培养基（命名为沙雷氏菌鉴别培养基）用于鉴别临床上常见的三种沙雷氏菌属菌种。加入抑制剂Irgasan DP - 300以消除大多数其余肠杆菌科细菌产生的假阳性反应。将疑似沙雷氏菌菌落穿刺接种到培养基中。粘质沙雷氏菌在培养18小时后颜色从橄榄色变为紫色，而深红沙雷氏菌（未列入《伯杰氏鉴定细菌学手册》）则变为亮黄色。液化沙雷氏菌（在《伯杰氏鉴定细菌学手册》[第8版，1974年]中描述为液化肠杆菌）在培养基顶部产生一条小的紫色带，底部为黄色或黄绿色。培养后无生长和颜色变化表明疑似菌落不是沙雷氏菌。对36株沙雷氏菌菌株以及97株其他肠杆菌科和假单胞菌科菌株进行了测试。两株非沙雷氏菌属的肠杆菌科细菌（弗氏柠檬酸杆菌和摩根氏变形杆菌各一株）以及两株铜绿假单胞菌在培养基中产生了颜色变化。使用该培养基对所有测试的沙雷氏菌菌株都进行了正确鉴定，而96%的其他测试菌种受到抑制。
