Department of Cardiology, Guangdong Provincial Hospital of Traditional Chinese Medicine; The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Bioengineered. 2021 Dec;12(2):10420-10429. doi: 10.1080/21655979.2021.1997224.
Atherosclerosis (AS) is a typical vascular disease. Emerging evidence has shown that circRNAs play key roles in the progression of AS, but the potential function and underlying mechanism of hsa_circ_0001879 remains unknown. We detected the expression level of hsa_circ_0001879 was determined by qRT-PCR, and the proliferation rate and migration ability of HUVECs were measured by CCK-8 assay and Transwell assay, respectively. Proliferative markers and epithelium mesenchymal transition (EMT) markers were measured through immunoblotting. A dual luciferase activity assay was performed to detect the interaction between circRNAs, miRNAs, and mRNAs. Hsa_circ_0001879 was upregulated in AS patients. Hsa_circ_0001879 inhibited the proliferation and migration ability of Human umbilical vein endothelial cells (HUVECs). Hsa_circ_0001879 directly bound to miR-6873-5p and acted as a sponge. miR-6873-5p-induced HDAC9 mRNA degradation was inhibited by hsa_circ_0001879. Hsa_circ_0001879 decreased the proliferation and migration of HUVECs by inhibiting miR-6873-5p-induced HDAC9 degradation.
动脉粥样硬化（AS）是一种典型的血管疾病。新出现的证据表明，circRNAs 在 AS 的进展中发挥着关键作用，但 hsa_circ_0001879 的潜在功能和作用机制尚不清楚。我们通过 qRT-PCR 检测 hsa_circ_0001879 的表达水平，通过 CCK-8 测定法和 Transwell 测定法分别测量 HUVECs 的增殖率和迁移能力。通过免疫印迹法测量增殖标志物和上皮间质转化（EMT）标志物。进行双荧光素酶活性测定以检测 circRNAs、miRNAs 和 mRNAs 之间的相互作用。hsa_circ_0001879 在 AS 患者中上调。hsa_circ_0001879 抑制人脐静脉内皮细胞（HUVECs）的增殖和迁移能力。hsa_circ_0001879 直接与 miR-6873-5p 结合并作为海绵。hsa_circ_0001879 抑制 miR-6873-5p 诱导的 HDAC9 mRNA 降解。hsa_circ_0001879 通过抑制 miR-6873-5p 诱导的 HDAC9 降解来降低 HUVECs 的增殖和迁移。
