转换后的高压锅和两个传统罐子用于厌氧细菌培养的性能

Performance of converted pressure cookers and two conventional jars for anaerobic bacterial culture.

作者信息

Gargan R A, Phillips I

出版信息

J Clin Pathol. 1978 May;31(5):426-9. doi: 10.1136/jcp.31.5.426.

DOI:10.1136/jcp.31.5.426

PMID:348726

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1145296/

Abstract

The simple conversion of commercial pressure cookers into inexpensive anaerobic jars is described. These containers were shown to be as good as the small conventional BBL polycarbonate GasPak and large vented 150 gas-replacement jars when assessed by means of three biological indicators: Pseudomonas aeruginosa, Bacteroides melaninogenicus, and Bacteroides fragilis. Ps. aeruginosa seeded on Simmond's citrate agar was shown to be the most sensitive indicator of the three for traces of oxygen.

摘要

本文描述了将商用高压锅简单改装为廉价厌氧罐的方法。通过三种生物指示剂：铜绿假单胞菌、产黑素拟杆菌和脆弱拟杆菌进行评估时，这些容器被证明与小型传统BBL聚碳酸酯气袋和大型带通风口的150气体置换罐一样好。接种在西蒙氏柠檬酸盐琼脂上的铜绿假单胞菌被证明是这三种指示剂中对微量氧气最敏感的。