Tally F P, Stewart P R, Sutter V L, Rosenblatt J E
J Clin Microbiol. 1975 Feb;1(2):161-4. doi: 10.1128/jcm.1.2.161-164.1975.
The oxygen tolerance and sensitivity of 57 freshly isolated anaerobic bacteria from clinical specimens was studied. All the organisms tolerated 8 h or more of exposure to oxygen in room air. Growth of the isolates in increasing oxygen concentrations demonstrated that the 57 isolates varied in oxygen sensitivity from strict to aerotolerant anaerobes. Comparison of the oxygen tolerance and sensitivity showed that the most tolerant organisms (best survival after prolonged exposure) included anaerobes capable of growth at only 0.4% or less O2 (strict) as well as those able to grow in as much as 10% O2. The least tolerant were predominately strict anaerobes. Decrease in the inoculum size from a concentration of 10(8) to 10(6) colony-forming units per ml had only a minor effect. The data indicate that the brief oxygen exposure with bench techniques in clinical laboratories would not be deleterious to the anaerobic bacteria present in clinical specimens.
对从临床标本中新鲜分离出的57株厌氧菌的氧耐受性和敏感性进行了研究。所有菌株在室温空气中耐受8小时或更长时间的氧气暴露。分离株在不断增加的氧气浓度下的生长表明，这57株分离株的氧敏感性从严格厌氧菌到兼性厌氧菌各不相同。氧耐受性和敏感性的比较表明，耐受性最强的生物体（长时间暴露后存活率最高）包括仅能在0.4%或更低氧气浓度下生长的厌氧菌（严格厌氧菌）以及能在高达10%氧气浓度下生长的厌氧菌。耐受性最差的主要是严格厌氧菌。接种量从每毫升10(8)个菌落形成单位降至10(6)个菌落形成单位的影响较小。数据表明，临床实验室中采用常规技术进行的短暂氧气暴露对临床标本中存在的厌氧菌无害。
