Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan.
Eur J Histochem. 2021 Dec 7;65(4):3237. doi: 10.4081/ejh.2021.3237.
Osteosarcoma (OS), characterized by high morbidity and mortality, is the most common bone malignancy worldwide. MicroRNAs (miRNAs) play a crucial role in the initiation and development of OS. The purpose of this study was to investigate the roles of miR-1270 in OS. RT-qPCR and Western blot were applied to detect the mRNA and protein level, respectively. CCK-8, colony formation, and TUNEL assays were conducted to determine the cell viability, proliferation, and apoptosis of OS cells. Wound healing and transwell assay were performed to detect the migration and invasion ability of OS cells. Bioinformatics analysis and dual-luciferase reporter assay were used to predict the target genes of miR-1270. Tumor xenograft in vivo assay was carried out to determine miR-1270 effect on the tumor size, volume, and weight. In this study, miR-1270 was overexpressed in OS tissues and cells. However, miR-1270 knockdown inhibited the proliferation, migration and invasion, and promoted the OS cells' apoptosis. Mechanistically, cingulin (CGN) was predicted and proved to be a target of miR-1270 and partially alleviated the effects of miR-1270 on the proliferation, migration and invasion ability of OS cells. Taken together, knockdown of miR-1270 may inhibit the development of OS via targeting CGN. This finding may provide a novel therapeutic strategy for OS.
骨肉瘤（OS）以高发病率和死亡率为特征，是世界范围内最常见的骨恶性肿瘤。microRNAs（miRNAs）在 OS 的发生和发展中起着至关重要的作用。本研究旨在探讨 miR-1270 在 OS 中的作用。应用 RT-qPCR 和 Western blot 分别检测 mRNA 和蛋白水平。CCK-8、集落形成和 TUNEL 测定分别用于检测 OS 细胞的活力、增殖和凋亡。划痕愈合和 Transwell 测定分别用于检测 OS 细胞的迁移和侵袭能力。生物信息学分析和双荧光素酶报告基因实验用于预测 miR-1270 的靶基因。体内肿瘤异种移植实验用于确定 miR-1270 对肿瘤大小、体积和重量的影响。在本研究中，miR-1270 在 OS 组织和细胞中过表达。然而，miR-1270 的下调抑制了 OS 细胞的增殖、迁移和侵袭，并促进了细胞凋亡。机制上，cingulin（CGN）被预测并证实是 miR-1270 的靶基因，部分缓解了 miR-1270 对 OS 细胞增殖、迁移和侵袭能力的影响。总之，下调 miR-1270 可能通过靶向 CGN 抑制 OS 的发展。这一发现可能为 OS 提供一种新的治疗策略。
