Chang Yun-Young, Lee SaYa, Jeong Hun-Jin, Cho Young-Sam, Lee Seung-Jae, Yun Jeong-Ho
Department of Dentistry, Inha International Medical Center, Incheon, Republic of Korea.
Department of Periodontology, College of Dentistry and Institute of Oral Bioscience, Jeonbuk National University, Jeonju, Republic of Korea.
J Biomed Mater Res B Appl Biomater. 2022 May;110(5):1103-1112. doi: 10.1002/jbm.b.34984. Epub 2021 Dec 7.
This study evaluated 3D printed polycaprolactone (PCL) composite scaffold and recombinant human bone morphogenetic protein-2 (rhBMP-2), loaded either onto a PCL composite scaffold or implant surface, for vertical bone augmentation with implant placement. Three-dimensional printed PCL frames were filled with powdered PCL, hydroxyapatite, and β-tricalcium phosphate. RhBMP-2 was loaded to the PCL composite scaffolds and implant surfaces, and rhBMP-2 release was quantified for 21 days. Experimental implants were placed bilaterally on 20 rabbit calvaria, and the PCL composite scaffolds were vertically augmented. The randomly allocated experimental groups were divided by carrier and rhBMP-2 dosage as no rhBMP-2 (control), 5 μg rhBMP-2 loaded to PCL composite (Scaffold/rhBMP-2[5 μg]), 5 μg rhBMP-2 loaded to implant (Implant/rhBMP-2[5 μg]), 30 μg rhBMP-2 loaded to PCL composite (Scaffold/rhBMP-2[30 μg]), and 30 μg rhBMP-2 loaded to implant (Implant/rhBMP-2[30 μg]). Histologic and histometric analyses were conducted after 8 weeks. In both scaffold-loading and implant-loading, rhBMP-2 released initially rapidly, then slowly and constantly. Released rhBMP-2 totaled 23.02 ± 1.03% and 24.69 ± 1.14% in the scaffold-loaded and implant-loaded groups, respectively. There were no significant differences in histologic bone-implant contact (%). Peri-implant bone density (%) was significantly higher in the Scaffold/rhBMP-2(30 μg) and Implant/rhBMP-2(30 μg) groups. Total bone density (%) was not significantly different between the Scaffold/rhBMP-2(5 μg), Implant/rhBMP-2(5 μg), and control groups, or between the Scaffold/rhBMP-2(30 μg) and Implant/rhBMP-2(30 μg) groups, but was significantly higher in the Scaffold/rhBMP-2(30 μg) and Implant/rhBMP-2(30 μg) groups than in the controls. Three-dimensional printed PCL composite scaffold with rhBMP-2 produced vertical osteogenesis and osseointegration, regardless of rhBMP-2 loading to the PCL composite scaffold or implant surface.
本研究评估了3D打印的聚己内酯（PCL）复合支架以及负载于PCL复合支架或种植体表面的重组人骨形态发生蛋白-2（rhBMP-2）在种植体植入时进行垂直骨增量的效果。3D打印的PCL框架填充有PCL粉末、羟基磷灰石和β-磷酸三钙。将rhBMP-2负载于PCL复合支架和种植体表面，并对rhBMP-2的释放进行了21天的定量分析。在20只兔颅骨双侧植入实验性种植体，并对PCL复合支架进行垂直增量。随机分配的实验组根据载体和rhBMP-2剂量分为：无rhBMP-2（对照组）、负载5μg rhBMP-2的PCL复合支架（支架/rhBMP-2[5μg]）、负载5μg rhBMP-2的种植体（种植体/rhBMP-2[5μg]）、负载30μg rhBMP-2的PCL复合支架（支架/rhBMP-2[30μg]）以及负载30μg rhBMP-2的种植体（种植体/rhBMP-2[30μg]）。8周后进行组织学和组织计量学分析。在支架负载组和种植体负载组中，rhBMP-2最初释放迅速，随后缓慢且持续释放。支架负载组和种植体负载组中rhBMP-2的总释放量分别为23.02±1.03%和24.69±1.14%。组织学骨-种植体接触率（%）无显著差异。支架/rhBMP-2（30μg）组和种植体/rhBMP-2（30μg）组的种植体周围骨密度（%）显著更高。支架/rhBMP-2（5μg）组、种植体/rhBMP-2（5μg）组与对照组之间，以及支架/rhBMP-2（30μg）组和种植体/rhBMP-2（30μg）组之间的总骨密度（%）无显著差异，但支架/rhBMP-2（30μg）组和种植体/rhBMP-2（30μg）组的总骨密度显著高于对照组。无论rhBMP-2是负载于PCL复合支架还是种植体表面，3D打印的含rhBMP-2的PCL复合支架均可产生垂直骨生成和骨整合。
