Zhou Pingping, Fang Xin, Xu Nannan, Yao Zhen, Xie Wenping, Ye Lidan
Joint International Research Laboratory of Agriculture and Agri-Product Safety, The Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
College of Bioscience and Biotechnology, Yangzhou University, Yangzhou, Jiangsu 225009, PR China.
ACS Synth Biol. 2021 Dec 17;10(12):3435-3444. doi: 10.1021/acssynbio.1c00378. Epub 2021 Dec 7.
Dynamic regulation of gene expression to decouple growth and production has been proven to be effective for improving the biosynthetic efficiency of microbial cell factories. However, the number of efficient regulatory systems available for regulation of is limited. In the present study, a novel copper-inducible gene expression system (CuIGR) composed of the copper-induced transcriptional activator Gal4 and the copper-inhibited repressor Gal80 was constructed in . When Gal80 was fused with a N-degron tag (K15), the resulting CuIGR4 system exhibited the most stringent regulation of gene expression driven by promoters. As compared to the native Cu-inducible promoter, the CuIGR4 system amplified the response to copper by as much as 2.7 folds, resulting in 72-fold induction of EGFP expression and a 33-fold change in lycopene production (3-100 mg/L) with addition of 20 μM copper. This newly developed copper-inducible system provides a powerful tool for gene expression control in , which is expected to be widely applicable in the regulation of yeast cell factories for enhanced biosynthesis of valuable products.
已证明动态调控基因表达以解耦生长与生产对于提高微生物细胞工厂的生物合成效率是有效的。然而，可用于调控的高效调控系统数量有限。在本研究中，在[具体生物]中构建了一种由铜诱导转录激活因子Gal4和铜抑制阻遏物Gal80组成的新型铜诱导基因表达系统（CuIGR）。当Gal80与N-降解标签（K15）融合时，所得的CuIGR4系统对由[具体启动子]启动子驱动的基因表达表现出最严格的调控。与天然铜诱导[具体启动子]相比，CuIGR4系统对铜的响应放大了2.7倍，在添加20μM铜的情况下，导致EGFP表达诱导72倍，番茄红素产量变化33倍（3 - 100mg/L）。这种新开发的铜诱导系统为[具体生物]中的基因表达控制提供了一个强大的工具，有望广泛应用于调控酵母细胞工厂以增强有价值产品的生物合成。
