Key Laboratory of Acupuncture of Tianjin, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion, Tianjin, 300381, China.
Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, 300381, China.
Chin J Integr Med. 2022 Feb;28(2):145-152. doi: 10.1007/s11655-021-3341-6. Epub 2021 Dec 7.
To investigate the effect of electro-acupuncture (EA) on vasomotor symptoms in rats with acute cerebral infarction, by observing the changes in the expression of factors related to the phosphatidylinositol (PI) system.
Forty-two Wistar rats were randomly divided into 3 groups by a random number table: the control group (n=6), the model group (n=18) and the EA group (n=18). The EA group was given EA treatment at Shuigou (GV 26) instantly after modeling with middle cerebral artery occlusion (MCAO) method, while the model and control groups were not given any treatment. The degrees of neurological deficiency were evaluated using neurological severity scores (NSS) and the brain blood flow was evaluated by a laser scanning confocal microscope. Western blot analysis was conducted to detect the expression levels of G-protein subtype (Gq) and calmodulin (CaM). Competition for protein binding was conducted to detect the expression level of inositol triphosphate (IP3). Thin layer quantitative analysis was conducted to detect the expression level of diacylglycerol (DAG). The expression level of intracellular concentration of free calcium ion ([Ca]) was detected by flow cytometry.
The NSS of the model group was significantly higher than the control group at 3 and 6 h after MCAO (P<0.01), while the EA group was significantly lower than the model group at 6 h (P<0.01). The cerebral blood flow in the model group was significantly lower than the control group at 1, 3 and 6 h after MCAO (P<0.01), while for the EA group it was remarkably higher than the model group at the same time points (P<0.01). The expressions of Gq, CaM, IP3, DAG and [Ca] in the model group were significantly higher than the control group (P<0.05 or P<0.01), and those in the EA group were significantly lower than the model group at the same time points (P<0.05 or P<0.01).
EA treatment at GV 26 can effectively decrease the over-expression of related factors of PI system in rats with acute cerebral infarction, improve cerebral autonomy movement, and alleviate cerebral vascular spasm.
通过观察磷脂酰肌醇（PI）系统相关因子表达的变化，探讨电针对急性脑梗死大鼠血管舒缩症状的影响。
42 只 Wistar 大鼠采用随机数字表法分为 3 组：对照组（n=6）、模型组（n=18）和电针组（n=18）。电针组采用大脑中动脉闭塞（MCAO）法造模后即刻给予水沟（GV 26）电针治疗，模型组和对照组不给予任何治疗。采用神经功能缺损评分（NSS）评估神经功能缺损程度，激光扫描共聚焦显微镜评估脑血流。Western blot 法检测 G 蛋白亚型（Gq）和钙调蛋白（CaM）的表达水平，竞争蛋白结合法检测三磷酸肌醇（IP3）的表达水平，薄层层析定量法检测二酰基甘油（DAG）的表达水平，流式细胞术检测细胞内游离钙离子浓度（[Ca]i）的表达水平。
MCAO 后 3、6 h 模型组 NSS 明显高于对照组（P<0.01），电针组明显低于模型组（P<0.01）；MCAO 后 1、3、6 h 模型组脑血流明显低于对照组（P<0.01），电针组明显高于模型组（P<0.01）。模型组 Gq、CaM、IP3、DAG 和[Ca]i 的表达明显高于对照组（P<0.05 或 P<0.01），电针组明显低于模型组（P<0.05 或 P<0.01）。
电针 GV 26 治疗可有效降低急性脑梗死大鼠 PI 系统相关因子的过度表达，改善脑自主运动，缓解脑血管痉挛。
