Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
DAIZ Inc., 3-14-3 Minami-kumamoto, Chuo-ku, Kumamoto 860-0812, Japan.
J Agric Food Chem. 2021 Dec 22;69(50):15165-15174. doi: 10.1021/acs.jafc.1c05208. Epub 2021 Dec 7.
Apart from the physiological effects of glyceollins, information regarding their tissue distribution is scarce in the literature. Thus, the aim of this study is to clarify the distribution of glyceollins in rat organs. Glyceollins I and III were orally administered to Sprague-Dawley rats (1.0 mg/kg) with daidzein as control, and their accumulations in organs were investigated by liquid chromatography-time-of-flight/mass spectrometry (LC-TOF/MS). Glyceollins accumulated in intact and conjugated forms in circulatory organs with a of 0.5 h, in the following order of descending preference: liver, kidney, heart, lung, soleus muscle, and abdominal aorta. The accumulation of hydrophobic glyceollin I was more than 1.5 times higher than that of III. In contrast, daidzein and hydroxy equol were detected only in the liver and kidneys at lower concentrations (1/100 times) than those of glyceollins. In conclusion, prenylated isoflavones, glyceollins, were preferentially distributed in circulatory organs as intact, sulfated, or glucuronidated forms up to 6 h after the intake.
除了 Glyceollins 的生理作用外，关于它们在组织中的分布的信息在文献中也很少。因此，本研究旨在阐明 Glyceollins 在大鼠器官中的分布。将 Glyceollins I 和 III 以黄豆苷元作为对照经口给予 Sprague-Dawley 大鼠（1.0mg/kg），并通过液相色谱-飞行时间/质谱联用（LC-TOF/MS）研究它们在器官中的积累。Glyceollins 以完整和结合形式在循环器官中积累，半衰期为 0.5 小时，以下降顺序为：肝、肾、心、肺、比目鱼肌和腹主动脉。疏水性 Glyceollin I 的积累量是 III 的 1.5 倍以上。相比之下，黄豆苷元和羟基雌马酚仅在肝脏和肾脏中以比 Glyceollins 低 100 倍的浓度检测到。总之，在摄入后 6 小时内，前体化异黄酮 Glyceollins 优先以完整的、硫酸化的或葡萄糖醛酸化的形式分布在循环器官中。
