Wondergem J, Begg A C, Haveman J
Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Jul;50(1):65-76. doi: 10.1080/09553008614550451.
The sensitivity of normal stroma to heat, irradiation and heat combined with irradiation has been studied using the tumour bed effect (TBE) assay. Irradiation before implantation led to a TBE. This TBE was dose dependent below 15 Gy, the TBE remaining relatively constant above 15 Gy. The interval (0-90 days) between irradiation and tumour implantation did not influence the magnitude of the TBE. Hyperthermia with large heat doses (45-60 min at 44 degrees C) before implantation may lead to a TBE. The interval between hyperthermia and tumour implantation proved to be very important. Our results show that the recovery from heat-induced stromal damage is very rapid. When the interval between hyperthermia and tumour implantation is 10 days or longer, no TBE could be observed. Irradiation combined with large heat doses (30-60 min at 44 degrees C) decreased the radiation-induced TBE. However, the combination of irradiation with mild heat treatments (15 min at 44 degrees C) could lead to a larger TBE than after irradiation alone. When hyperthermia was given prior to irradiation, the interval between heat and irradiation proved to be very important. With large intervals (21 days or longer) the TBE values were about the same as with irradiation alone. When heat was given after irradiation it always reduced the irradiation-induced TBE.
利用肿瘤床效应（TBE）分析法，研究了正常基质对热、辐射以及热与辐射联合作用的敏感性。植入前进行辐射会导致肿瘤床效应。在15 Gy以下，这种肿瘤床效应呈剂量依赖性，在15 Gy以上时，肿瘤床效应相对保持恒定。辐射与肿瘤植入之间的间隔时间（0 - 90天）并不影响肿瘤床效应的大小。植入前给予大剂量热疗（44℃下45 - 60分钟）可能会导致肿瘤床效应。热疗与肿瘤植入之间的间隔时间被证明非常重要。我们的结果表明，热诱导的基质损伤恢复非常迅速。当热疗与肿瘤植入之间的间隔时间为10天或更长时，未观察到肿瘤床效应。辐射与大剂量热疗（44℃下30 - 60分钟）联合使用会降低辐射诱导的肿瘤床效应。然而，辐射与温和热疗（44℃下15分钟）联合使用可能会导致比单独辐射后更大的肿瘤床效应。当在辐射前进行热疗时，热疗与辐射之间的间隔时间被证明非常重要。间隔时间长（21天或更长）时，肿瘤床效应值与单独辐射时大致相同。当在辐射后进行热疗时，它总是会降低辐射诱导的肿瘤床效应。
