Hill S A, Smith K A, Denekamp J
Cancer Research Campaign, Gray Laboratory, Mount Vernon Hospital, Northwood, Middlesex, UK.
Br J Cancer. 1987 Oct;56(4):383-8. doi: 10.1038/bjc.1987.211.
The influence on stroma of heat alone, X-rays alone or the combined treatment, has been studied using the tumour bed effect (TBE) as an assay. Ca NT cells have been implanted into previously treated subcutaneous sites as an angiogenic stimulus. The vascular damage is then assessed by the reduced tumour growth rate, which results from inadequate vascular proliferation. A range of X-ray doses was used and large alterations in latent period for growth to 2 mm diameter were followed by smaller alterations in the growth rate of established tumours. A dose response relationship was seen for latency (0-20 Gy) and for growth rate (0-16 Gy). A range of subcutaneous temperatures was obtained by immersion in a water bath for 60 minutes at 40 degrees, 41.5 degrees, 43 degrees or 44.5 degrees C. A slight retardation of tumour growth was seen after 41.5 degrees C, but an unexpected acceleration resulted from the highest heat treatment. Combined heat and X-ray treatments showed thermal sensitization of the X-ray induced TBE at 41.5 degrees C, with a reversal at higher temperatures. At 43 degrees C and 44.5 degrees C a mild thermal burn was induced and this appeared to elicit neovascularisation that could be utilized by the implanted tumour cells. Delayed implantation of tumour cells (at 4 weeks instead of 1 day) abolished this effect.
利用肿瘤床效应（TBE）作为一种检测方法，研究了单独加热、单独使用X射线或联合治疗对基质的影响。将Ca NT细胞植入先前处理过的皮下部位作为血管生成刺激。然后通过肿瘤生长速率降低来评估血管损伤，这是由血管增殖不足导致的。使用了一系列X射线剂量，生长至直径2毫米的潜伏期有较大变化，随后已形成肿瘤的生长速率有较小变化。在潜伏期（0 - 20 Gy）和生长速率（0 - 16 Gy）方面都观察到了剂量反应关系。通过在40℃、41.5℃、43℃或44.5℃的水浴中浸泡60分钟获得了一系列皮下温度。在41.5℃后观察到肿瘤生长略有迟缓，但最高温度处理导致了意想不到的加速。热与X射线联合治疗显示在41.5℃时X射线诱导的TBE出现热增敏，在较高温度下则出现逆转。在43℃和44.5℃时诱导了轻度热灼伤，这似乎引发了新血管形成，植入的肿瘤细胞可以利用这些新血管。延迟植入肿瘤细胞（4周而不是1天）消除了这种效应。
