Isakson P C, Krolick K A, Uhr J W, Vitetta E S
J Immunol. 1980 Aug;125(2):886-92.
In these studies, we have compared the effects of anti-mu and anti-delta antibodies on normal and neoplastic murine B cells. Spleen cells from normal BALB/c mice or from mice carrying the BCL1 tumor were treated with F(ab')2 fragments of affinity purified anti-mu or anti-delta antibodies and the effec of the antibodies on cell proliferation was assessed. F(ab)'2 fragments of both antibodies induced proliferation of normal adult B cells but inhibited (anti-mu) or had no effect (anti-delta) on proliferation of BCL1 cells. In addition, both antibodies further enhanced the LPS-induced proliferative response of cells from adult mice. In contrast, the LPS responses of neonatal cells and BCL1 cells were inhibited by F(ab')2 anti-mu antibodies. These data indicate that interaction of antibody with surface IgD as well as surface IgM can activate adult B cells. In addition, the results provide further evidence that BCL1 cells are neoplastic analogs of immature B cells since the tumor cells, like neonatal cells, are not activated after treatment with anti-Ig antibodies.
在这些研究中，我们比较了抗μ和抗δ抗体对正常和肿瘤性小鼠B细胞的作用。用亲和纯化的抗μ或抗δ抗体的F(ab')2片段处理来自正常BALB/c小鼠或携带BCL1肿瘤的小鼠的脾细胞，并评估抗体对细胞增殖的影响。两种抗体的F(ab')2片段均诱导正常成年B细胞增殖，但对BCL1细胞的增殖有抑制作用（抗μ）或无影响（抗δ）。此外，两种抗体均进一步增强了成年小鼠细胞的LPS诱导的增殖反应。相反，新生细胞和BCL1细胞的LPS反应被F(ab')2抗μ抗体抑制。这些数据表明抗体与表面IgD以及表面IgM的相互作用可激活成年B细胞。此外，结果提供了进一步的证据，即BCL1细胞是未成熟B细胞的肿瘤类似物，因为肿瘤细胞与新生细胞一样，在用抗Ig抗体处理后未被激活。
