Istanbul Faculty of Medicine, Department of Histology and Embryology, Istanbul University, İstanbul, Turkey.
Graduate School of Health Sciences, Istanbul University, İstanbul, Turkey.
Ultrastruct Pathol. 2021 Nov 2;45(6):384-390. doi: 10.1080/01913123.2021.1987605.
Monobenzyl ether of hydroquinone (MBEH) is a topical depigmentation agent used by vitiligo patients to even the skin tone. We aimed to investigate the effects of MBEH on 3T3 mouse fibroblasts. Fibroblasts were treated with 250 µM, 500 µM, and 750 µM MBEH and vehicle (EtOH:DMSO) for 24 hours. Cell numbers of 250 µM, 500 µM, and 750 µM MBEH treated and vehicle groups decreased significantly compared to control group. TUNEL positive cell rate increased with MBEH concentration. In electron microscopic examination, control and vehicle groups showed active cells features, while mitochondrial swelling and cristae loss were seen in 250 µM MBEH-treated group. In cytoplasm of 500 µM MBEH-treated group, there were many multivesicular bodies and autophagic vacuoles. As an indication of apoptosis, cell membrane blebs and reduction in cell size were observed. In 750 μM MBEH-treated group, cells were completely degenerated. Our findings show that MBEH, which is used as a depigmentation agent to lighten the skin by destroying melanocytes, may also have dose-dependent negative effects on the viability of 3T3 mouse fibroblasts, and these may be mediated through autophagic and apoptotic cell death mechanisms.
对苯二酚单苄醚 (MBEH) 是一种局部脱色剂，用于治疗白癜风患者以均匀肤色。我们旨在研究 MBEH 对 3T3 小鼠成纤维细胞的影响。用 250µM、500µM 和 750µM MBEH 及载体（EtOH:DMSO）处理成纤维细胞 24 小时。与对照组相比，250µM、500µM 和 750µM MBEH 处理组和载体组的细胞数量明显减少。随着 MBEH 浓度的增加，TUNEL 阳性细胞率增加。在电子显微镜检查中，对照组和载体组显示出活跃的细胞特征，而在 250µM MBEH 处理组中可见线粒体肿胀和嵴丢失。在 500µM MBEH 处理组的细胞质中，有许多多泡体和自噬空泡。作为凋亡的一个指标，观察到细胞膜泡和细胞体积减小。在 750μM MBEH 处理组中，细胞完全退化。我们的研究结果表明，MBEH 作为一种脱色剂，通过破坏黑色素细胞来使皮肤变浅，可能对 3T3 小鼠成纤维细胞的活力也有剂量依赖性的负作用，这可能是通过自噬和凋亡细胞死亡机制介导的。
