Laboratory of Radiobiology and Mutagenesis, Institute of Biological Sciences, Federal University of Goiás, Goiânia, Brazil.
Laboratory of Biotechnology, Henrique Santillo Campus, State University of Goiás, Anápolis, Brazil.
J Toxicol Environ Health A. 2022 May 3;85(9):353-363. doi: 10.1080/15287394.2021.2009947. Epub 2021 Dec 7.
Pedunculagin (PD), an ellagitannin found in different plant species, possesses several pharmaceutical properties, including antitumor, antioxidant, gastroprotective, hepatoprotective, and anti-inflammatory properties. However, the effects of PD alone on DNA remain to be determined. The aim of this study was to investigate the potential cytotoxic, genotoxic, and antigenotoxic activities of PD isolated from seeds using and assays. To elucidate the biological activities of PD, tools indicative of antioxidant, antineoplastic, and chemopreventive activities of PD were used. Subsequently, the mutagenic/antimutagenic effects of PD were later assessed using bacteria with the Ames test, and the cytotoxic, genotoxic, and antigenotoxic effects utilizing human lymphocytes as evidenced by trypan blue exclusion test and CometChip assay. analysis indicated potential antioxidant, chemopreventive, free radical scavenger, and cytostatic activities of PD. In the Ames test, PD was found to be not mutagenic; however, this plant component protected DNA against damage-mediated by mutagens 4-nitroquinoline-1-oxide and sodium azide. Regarding human lymphocytes, PD alone was cytotoxic and genotoxic; however, it also reduced DNA damage induced by doxorubicin at co- and post-treatment. In conclusion, PD showed genotoxic, antigenotoxic and cytotoxic effects in human lymphocytes and antimutagenic effects in bacteria.
五羟黄酮（PD）是一种存在于不同植物物种中的鞣花单宁，具有多种药物特性，包括抗肿瘤、抗氧化、胃保护、肝保护和抗炎特性。然而，PD 单独对 DNA 的影响仍有待确定。本研究旨在使用和试验研究从种子中分离出的 PD 对 DNA 的潜在细胞毒性、遗传毒性和抗原毒性活性。为了阐明 PD 的生物学活性，使用了抗氧化、抗肿瘤和化学预防活性的工具来评估 PD 的作用。随后，使用具有 Ames 试验的细菌评估 PD 的诱变/抗诱变作用，使用台盼蓝排除试验和彗星芯片试验利用人淋巴细胞评估 PD 的细胞毒性、遗传毒性和抗原毒性作用。分析表明 PD 具有潜在的抗氧化、化学预防、自由基清除和细胞抑制活性。在 Ames 试验中，PD 未被发现具有致突变性；然而，这种植物成分可保护 DNA 免受 4-硝基喹啉-1-氧化物和叠氮化钠介导的损伤。关于人淋巴细胞，PD 单独具有细胞毒性和遗传毒性；然而，它还可以减少阿霉素在共同和后续处理时引起的 DNA 损伤。总之，PD 在人淋巴细胞中显示出遗传毒性、抗原毒性和细胞毒性作用，在细菌中显示出抗诱变作用。
