Laboratory of Biology and Biotechnology of Microorganisms, Department of Biology, Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000, Marrakech, Morocco.
World J Microbiol Biotechnol. 2012 Jan;28(1):303-11. doi: 10.1007/s11274-011-0820-5. Epub 2011 Jun 23.
Pectobacterium carotovorum and Pectobacterium atrosepticum are dreadful causal agents of potato soft rot. Actually, there are no efficient bactericides used to protect potato against Pectobacterium spp. Biological control using actinobacteria could be an interesting approach to manage this disease. Thus, two hundred actinobacteria isolated from Moroccan habitats were tested for their ability to inhibit in vitro 4 environmental Pectobacterium strains and the two reference strains (P. carotovorum CFBP 5890 and P. atrosepticum CFBP 5889). Eight percent of these isolates were active against at least one of the tested pathogens and only 2% exhibited an antimicrobial activity against all tested Pectobacterium strains. Four bioactive isolates having the greatest pathogen inhibitory capabilities and classified as belonging to the genus Streptomyces species through 16S rDNA analysis were subsequently tested for their ability to reduce in vivo soft rot symptoms on potato slices of Bintje, Yukon Gold, Russet and Norland cultivars caused by the two pathogens P. carotovorum and P. atrosepticum. This test was carried out by using biomass inoculums and culture filtrate of the isolates as treatment. Among these, strain Streptomyces sp. OE7, reduced by 65-94% symptom severity caused by the two pathogens on potato slices. Streptomyces OE7 showed a potential for controlling soft rot on potato slices and could be useful in an integrated control program against potato soft rot pathogens in the objective to reduce treatments with chemical compounds.
胡萝卜软腐果胶杆菌和腐烂果胶杆菌是马铃薯软腐病的可怕病原体。实际上，没有有效的杀菌剂可用于保护马铃薯免受果胶杆菌属的侵害。使用放线菌进行生物防治可能是管理这种疾病的一种有趣方法。因此，从摩洛哥生境中分离出的 200 株放线菌被测试其抑制体外 4 种环境果胶杆菌菌株和 2 种参考菌株（胡萝卜软腐果胶杆菌 CFBP 5890 和腐烂果胶杆菌 CFBP 5889）的能力。这些分离物中有 8%至少对一种测试病原体具有活性，只有 2%对所有测试的果胶杆菌菌株表现出抗菌活性。通过 16S rDNA 分析，随后对具有最大病原体抑制能力的 4 种具有生物活性的分离物进行测试，以评估它们在活体条件下降低由两种病原体胡萝卜软腐果胶杆菌和腐烂果胶杆菌引起的 Bintje、Yukon Gold、Russet 和 Norland 品种马铃薯切片软腐病症状的能力。通过使用生物量接种物和分离物的培养液进行了该测试。其中，菌株链霉菌 OE7 将两种病原体引起的马铃薯切片的症状严重程度降低了 65-94%。链霉菌 OE7 显示出对马铃薯切片软腐病的控制潜力，可用于减少化学化合物处理的综合防治计划中，以防治马铃薯软腐病病原体。
