辅助性T细胞和杀伤性T细胞不表达B细胞免疫球蛋白连接区和恒定区基因片段。
Helper and killer T cells do not express B cell immunoglobulin joining and constant region gene segments.
作者信息
Kronenberg M, Davis M M, Early P W, Hood L E, Watson J D
出版信息
J Exp Med. 1980 Dec 1;152(6):1745-61. doi: 10.1084/jem.152.6.1745.
Abstract
We have analyzed four kinds of T cells for rearrangement and expression of immunoglobulin genes. These cells include: (a) whole thymus; (b) WEHI-22, a T-cell lymphoma; (c) HT-1, an major histocompatibility complex-restricted T helper line; and (d) CTLLi6, an H-2 alloreactive killer cell line. None of the B-cell joining and constant gene segments are rearranged in the T cells. The monoclonal cells do not express any C kappa, C lambda, Cmu or C alpha RNA species. Small amounts of C kappa, C alpha, and Cmu sequences are present in RNA prepared from the thymus, although the significance of this RNA for T-cell antigen receptor synthesis is uncertain. The data support the hypothesis that expression of B-cell joining and C gene segments is unnecessary for T-cell helper and T-cell killer activity.
摘要
我们分析了四种T细胞中免疫球蛋白基因的重排和表达情况。这些细胞包括：（a）整个胸腺；（b）WEHI-22，一种T细胞淋巴瘤；（c）HT-1，一种主要组织相容性复合体限制的T辅助细胞系；以及（d）CTLLi6，一种H-2同种异体反应性杀伤细胞系。在T细胞中，没有B细胞连接区和恒定基因片段发生重排。这些单克隆细胞不表达任何Cκ、Cλ、Cμ或CαRNA种类。从胸腺制备的RNA中存在少量的Cκ、Cα和Cμ序列，尽管这种RNA对T细胞抗原受体合成的意义尚不确定。这些数据支持这样一种假说，即T细胞辅助活性和T细胞杀伤活性不需要B细胞连接区和C基因片段的表达。
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Helper and killer T cells do not express B cell immunoglobulin joining and constant region gene segments.辅助性T细胞和杀伤性T细胞不表达B细胞免疫球蛋白连接区和恒定区基因片段。
J Exp Med. 1980 Dec 1;152(6):1745-61. doi: 10.1084/jem.152.6.1745.
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本文引用的文献
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Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.产生完整的免疫球蛋白重链基因需要两种类型的体细胞重组。
Nature. 1980 Aug 14;286(5774):676-83. doi: 10.1038/286676a0.
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The immunoglobulin mu chains of membrane-bound and secreted IgM molecules differ in their C-terminal segments.膜结合型和分泌型IgM分子的免疫球蛋白μ链在其C末端片段上有所不同。
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Deletions in the constant region locus can account for switches in immunoglobulin heavy chain expression.恒定区基因座的缺失可解释免疫球蛋白重链表达的转换。
Nature. 1980 Jun 12;285(5765):450-6. doi: 10.1038/285450a0.
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Expression of the immunoglobulin C mu gene in mouse T and B lymphoid and myeloid cell lines.免疫球蛋白Cμ基因在小鼠T和B淋巴细胞系及髓细胞系中的表达。
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Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.通过可变RNA加工途径，可从单个免疫球蛋白μ基因产生两种mRNA。
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Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.两个具有不同3'末端的mRNA编码免疫球蛋白μ链的膜结合形式和分泌形式。
Cell. 1980 Jun;20(2):303-12. doi: 10.1016/0092-8674(80)90616-9.
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An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.免疫球蛋白重链可变区基因由三段DNA片段组成：VH、D和JH。
Cell. 1980 Apr;19(4):981-92. doi: 10.1016/0092-8674(80)90089-6.
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Rearrangement of immunoglobulin gamma 1-chain gene and mechanism for heavy-chain class switch.免疫球蛋白γ1链基因重排及重链类别转换机制
Proc Natl Acad Sci U S A. 1980 Feb;77(2):919-23. doi: 10.1073/pnas.77.2.919.
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Nature. 1980 Feb 21;283(5749):733-9. doi: 10.1038/283733a0.
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