人白细胞介素1两种阴离子形式的纯化至均一性及氨基酸序列分析
Purification to homogeneity and amino acid sequence analysis of two anionic species of human interleukin 1.
作者信息
Cameron P M, Limjuco G A, Chin J, Silberstein L, Schmidt J A
出版信息
J Exp Med. 1986 Jul 1;164(1):237-50. doi: 10.1084/jem.164.1.237.
Abstract
Two anionic species of human IL-1 have been purified to homogeneity. These molecules were characterized as having pI of 5.4 and 5.2 and molecular weights identical to IL-1/6.8 (17,500). The specific activities of IL-1/5.4 and IL-1/5.2, as measured in the mouse thymocyte co-mitogenic assay, were identical to that of IL-1/6.8, namely 1.2 X 10(7) U/mg, with half-maximal stimulation observed at 2 X 10(-11) M. IL-1/5.4 and IL-1/5.2 were found to be antigenically distinct from IL-1/6.8 in an ELISA. IL-1/5.4 was structurally distinct from IL-1/6.8 based on reverse-phase HPLC or CNBr peptides. Intact IL-1/5.2 and three intact CNBr peptides of IL-1/5.4 were sequenced, with the identification of 74 amino acid residues. These sequences were found to correspond exactly with the amino acid sequence deduced from the IL-1-alpha cDNA reported by March et al.
摘要
已将人白细胞介素-1的两种阴离子形式纯化至同质。这些分子的特征为其等电点分别为5.4和5.2，分子量与白细胞介素-1/6.8（17,500）相同。在小鼠胸腺细胞共促有丝分裂试验中测得的白细胞介素-1/5.4和白细胞介素-1/5.2的比活性与白细胞介素-1/6.8相同，即1.2×10⁷U/mg，在2×10⁻¹¹M时观察到半数最大刺激。在酶联免疫吸附测定中发现白细胞介素-1/5.4和白细胞介素-1/5.2在抗原性上与白细胞介素-1/6.8不同。基于反相高效液相色谱法或溴化氰肽，白细胞介素-1/5.4在结构上与白细胞介素-1/6.8不同。测定了完整的白细胞介素-1/5.2和白细胞介素-1/5.4的三个完整溴化氰肽的序列，鉴定出74个氨基酸残基。发现这些序列与March等人报道的白细胞介素-1-α cDNA推导的氨基酸序列完全一致。
相似文献
1
Purification to homogeneity and amino acid sequence analysis of two anionic species of human interleukin 1.人白细胞介素1两种阴离子形式的纯化至均一性及氨基酸序列分析
J Exp Med. 1986 Jul 1;164(1):237-50. doi: 10.1084/jem.164.1.237.
2
Amino acid sequence analysis of human interleukin 1 (IL-1). Evidence for biochemically distinct forms of IL-1.人白细胞介素1（IL-1）的氨基酸序列分析。IL-1存在生化特性不同形式的证据。
J Exp Med. 1985 Sep 1;162(3):790-801. doi: 10.1084/jem.162.3.790.
3
Purification and biochemical characteristics of two distinct human interleukins 1 from the myelomonocytic THP-1 cell line.从骨髓单核细胞THP-1细胞系中纯化两种不同的人白细胞介素1及其生化特性
Biochemistry. 1986 Jun 3;25(11):3424-9. doi: 10.1021/bi00359a049.
4
Purification and partial biochemical characterization of normal human interleukin 1.正常人白细胞介素1的纯化及部分生化特性分析
J Exp Med. 1984 Sep 1;160(3):772-87. doi: 10.1084/jem.160.3.772.
5
Human interleukin 1. Purification to homogeneity.人白细胞介素1。纯化至均一性。
J Exp Med. 1985 Mar 1;161(3):490-502. doi: 10.1084/jem.161.3.490.
6
Comparison of biological and immunological activities of human monocyte-derived interleukin 1 beta and human recombinant interleukin 1 beta.
Scand J Immunol. 1990 Feb;31(2):225-35. doi: 10.1111/j.1365-3083.1990.tb02763.x.
7
8
Pig interleukin 1. Purification of two immunologically different leukocyte proteins that cause cartilage resorption, lymphocyte activation, and fever.猪白细胞介素1。两种免疫上不同的白细胞蛋白的纯化，这两种蛋白可导致软骨吸收、淋巴细胞活化和发热。
J Exp Med. 1985 Oct 1;162(4):1208-22. doi: 10.1084/jem.162.4.1208.
9
Purification and characterization of recombinant human interleukin-1 beta produced in Escherichia coli.在大肠杆菌中产生的重组人白细胞介素-1β的纯化与特性鉴定
Biochem Biophys Res Commun. 1987 Aug 31;147(1):315-21. doi: 10.1016/s0006-291x(87)80123-7.
10
Enhanced immunogenicity of a sequence derived from hepatitis B virus surface antigen in a composite peptide that includes the immunostimulatory region from human interleukin 1.源自乙肝病毒表面抗原的序列在包含人白细胞介素1免疫刺激区的复合肽中的免疫原性增强。
Proc Natl Acad Sci U S A. 1990 Jul;87(14):5519-22. doi: 10.1073/pnas.87.14.5519.
引用本文的文献
1
Early circulating erythroid progenitors (BFU-E) in sickle cell anemia.镰状细胞贫血中的早期循环红系祖细胞（爆式红系集落形成单位）
Experientia. 1993 Feb 15;49(2):118-25. doi: 10.1007/BF01989415.
2
Specific bioactivities of monocyte-derived interleukin 1 alpha and interleukin 1 beta are similar to each other on cultured murine thymocytes and on cultured human connective tissue cells.单核细胞衍生的白细胞介素1α和白细胞介素1β在培养的小鼠胸腺细胞和培养的人结缔组织细胞上的特定生物活性彼此相似。
J Clin Invest. 1986 Sep;78(3):836-9. doi: 10.1172/JCI112649.
3
The molecular forms of interleukin-1.
Immunol Res. 1986;5(4):271-80. doi: 10.1007/BF02935500.
4
Interleukin 1 beta is localized in the cytoplasmic ground substance but is largely absent from the Golgi apparatus and plasma membranes of stimulated human monocytes.白细胞介素1β定位于细胞质基质中，但在受刺激的人单核细胞的高尔基体和质膜中基本不存在。
J Exp Med. 1988 Feb 1;167(2):389-407. doi: 10.1084/jem.167.2.389.
5
The role of tumor necrosis factor and interleukin 1 in the immunoinflammatory response.肿瘤坏死因子和白细胞介素1在免疫炎症反应中的作用。
Pharm Res. 1988 Mar;5(3):129-39. doi: 10.1023/a:1015904721223.
6
Identification of a high-affinity receptor for interleukin 1 alpha and interleukin 1 beta on cultured human rheumatoid synovial cells.
J Clin Invest. 1988 Aug;82(2):420-6. doi: 10.1172/JCI113614.
7
Determination of the minimum polypeptide lengths of the functionally active sites of human interleukins 1 alpha and 1 beta.
Proc Natl Acad Sci U S A. 1987 Jul;84(13):4572-6. doi: 10.1073/pnas.84.13.4572.
8
Identification of a high-affinity receptor for native human interleukin 1 beta and interleukin 1 alpha on normal human lung fibroblasts.正常人肺成纤维细胞上天然人白细胞介素1β和白细胞介素1α高亲和力受体的鉴定
J Exp Med. 1987 Jan 1;165(1):70-86. doi: 10.1084/jem.165.1.70.
9
Epstein-Barr virus-containing epithelial cells from nasopharyngeal carcinoma produce interleukin 1 alpha.
Proc Natl Acad Sci U S A. 1987 Sep;84(17):6262-6. doi: 10.1073/pnas.84.17.6262.
10
Release of interleukin-1 beta associated with potent cytocidal action of staphylococcal alpha-toxin on human monocytes.白细胞介素-1β的释放与葡萄球菌α毒素对人单核细胞的强效杀细胞作用相关。
Infect Immun. 1989 Nov;57(11):3512-9. doi: 10.1128/iai.57.11.3512-3519.1989.
本文引用的文献
1
Purification to apparent homogeneity of murine interleukin 1.将小鼠白细胞介素1纯化至表观均一性。
J Immunol. 1981 Mar;126(3):834-7.
2
Purification to homogeneity of pig leucocyte catabolin, a protein that causes cartilage resorption in vitro.猪白细胞分解代谢素（一种在体外可引起软骨吸收的蛋白质）的纯化至均一性。
Biochem J. 1983 Nov 1;215(2):385-92. doi: 10.1042/bj2150385.
3
Interleukin 1, a potential regulator of fibroblast proliferation.白细胞介素1，一种成纤维细胞增殖的潜在调节因子。
J Immunol. 1982 May;128(5):2177-82.
4
Purification to homogeneity of biologically active human interleukin 1.生物活性人白细胞介素1的纯化至均一性。
Prep Biochem. 1984;14(5):449-70. doi: 10.1080/00327488408061780.
5
Structural analysis of the charge heterogeneity of human interleukin 1.人白细胞介素1电荷异质性的结构分析
Arch Biochem Biophys. 1984 Nov 1;234(2):371-6. doi: 10.1016/0003-9861(84)90282-0.
6
Purification of human interleukin 1 by high-performance liquid chromatography.用高效液相色谱法纯化人白细胞介素1
J Chromatogr. 1984 Jul 27;296:293-300. doi: 10.1016/s0021-9673(01)96423-4.
7
Purification and partial biochemical characterization of normal human interleukin 1.正常人白细胞介素1的纯化及部分生化特性分析
J Exp Med. 1984 Sep 1;160(3):772-87. doi: 10.1084/jem.160.3.772.
8
Cloning and expression of murine interleukin-1 cDNA in Escherichia coli.小鼠白细胞介素-1 cDNA在大肠杆菌中的克隆与表达。
Nature. 1984;312(5993):458-62. doi: 10.1038/312458a0.
9
Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels.十二烷基硫酸钠-聚丙烯酰胺凝胶的蛋白质污染物
Anal Biochem. 1983 Dec;135(2):470-4. doi: 10.1016/0003-2697(83)90714-5.
10
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.聚丙烯酰胺凝胶中蛋白质的银染：一种具有增强均匀灵敏度的改良方法。
Anal Biochem. 1981 Nov 1;117(2):307-10. doi: 10.1016/0003-2697(81)90783-1.
Zotero 插件