正常人白细胞介素1的纯化及部分生化特性分析
Purification and partial biochemical characterization of normal human interleukin 1.
作者信息
Schmidt J A
出版信息
J Exp Med. 1984 Sep 1;160(3):772-87. doi: 10.1084/jem.160.3.772.
Abstract
A protocol for the rapid, efficient purification of the major charged species of human interleukin 1 (IL-1) has been developed using high performance anion exchange and size exclusion chromatography. The isolated material is pure as determined by sodium dodecyl sulfate (SDS) gradient polyacrylamide gel electrophoresis (PAGE) and analytical isoelectric focusing (IEF). The molecular weight of the purified material is 15,000 and the isoelectric point (pI) is 6.8, values that are in good agreement with those previously reported for human IL-1. 10(-10) M concentrations of the purified material give half-maximal stimulation in the thymocyte proliferation assay. Amounts of IL-1 sufficient for receptor studies and detailed biochemical analysis can now be produced on a regular basis.
摘要
已开发出一种使用高效阴离子交换色谱和尺寸排阻色谱快速、高效纯化人白细胞介素1（IL-1）主要带电形式的方法。通过十二烷基硫酸钠（SDS）梯度聚丙烯酰胺凝胶电泳（PAGE）和分析等电聚焦（IEF）测定，分离得到的物质是纯的。纯化物质的分子量为15,000，等电点（pI）为6.8，这些值与先前报道的人IL-1的值高度一致。在胸腺细胞增殖试验中，纯化物质浓度为10(-10) M时产生半数最大刺激。现在可以定期生产足以用于受体研究和详细生化分析的IL-1量。
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