Alvi Roman Farooq, Aslam Bilal, Rasool Muhammad Hidayat, Muzammil Saima, Siddique Abu Baker, Yasmeen Nafeesa, Khurshid Mohsin, Sarwar Noreen, Almatroudi Ahmad, Hussain Riaz, Baloch Zulqarnain
Department of Microbiology, Government College University Faisalabad, Faisalabad, Pakistan.
College of Veterinary Medicine, South China Agricultural University, Guangzhou 610642, China.
Can J Infect Dis Med Microbiol. 2021 Nov 28;2021:5570963. doi: 10.1155/2021/5570963. eCollection 2021.
The term "persisters" refers to a small bacterial population that persists during treatment with high antibiotic concentration or dose in the absence of genetic resistance. The present study was designed to investigate the transcriptional response in indigenous under the ciprofloxacin stress.
Isolation and identification of were carried out through standard microbiological protocols. The characterization of quinolone resistance was performed by estimating the quinolone susceptibility testing, MIC estimation, and detecting the QRDR and PMQR. Transcriptional response of the isolates to ciprofloxacin was determined using qPCR.
Among 34 isolates, 23 (67%) were resistant to ciprofloxacin. Both QRDR ( and ) and PMQR ( and ) were detected in the isolates, and all were found resistant to ciprofloxacin. The mRNA levels of both and under the influence of ciprofloxacin were significantly increased. On ciprofloxacin exposure, the mRNA levels of the DNA damage response element () were raised in a time-dependent fashion. showed high-level resistance to ciprofloxacin in the presence of mutations in QRDR and PMQR genes.
The transcriptional response revealed the upregulation of DNA repair and protein folding elements ( and ) in ciprofloxacin stress and delayed cell division. The ciprofloxacin was found to trigger various stress responses in a time- and concentration-dependent manner.
“持留菌”一词指的是在不存在遗传抗性的情况下，在高抗生素浓度或剂量治疗期间持续存在的一小部分细菌群体。本研究旨在调查环丙沙星应激下本土细菌的转录反应。
通过标准微生物学方案进行细菌的分离和鉴定。通过评估喹诺酮敏感性试验、最低抑菌浓度（MIC）估计以及检测喹诺酮耐药决定区（QRDR）和质粒介导的喹诺酮耐药（PMQR）来进行喹诺酮耐药性的表征。使用定量聚合酶链反应（qPCR）确定分离株对环丙沙星的转录反应。
在34株分离株中，23株（67%）对环丙沙星耐药。在分离株中检测到QRDR（[具体基因1]和[具体基因2]）和PMQR（[具体基因3]和[具体基因4]），并且所有分离株均对环丙沙星耐药。在环丙沙星影响下，[具体基因5]和[具体基因6]的mRNA水平均显著升高。在环丙沙星暴露时，DNA损伤反应元件（[具体元件名称]）的mRNA水平呈时间依赖性升高。在QRDR和PMQR基因存在突变的情况下，[具体菌株名称]对环丙沙星表现出高水平耐药。
转录反应揭示了在环丙沙星应激下DNA修复和蛋白质折叠元件（[具体基因5]和[具体基因6]）的上调以及细胞分裂延迟。发现环丙沙星以时间和浓度依赖性方式触发各种应激反应。
