Srikanth Sujata, Cascio Lauren, Pauly Rini, Jones Kelly, Sorrow Skylar, Cubillan Rossana, Chen Chin-Fu, Skinner Cindy D, Champaigne Kevin, Stevenson Roger E, Schwartz Charles E, Boccuto Luigi
Greenwood Genetic Center, 101 Gregor Mendel Circle, Greenwood, SC 29646, USA.
Circa Bioscience, Greenwood, 113 Gregor Mendel Circle, Greenwood, SC 29646, USA.
Data Brief. 2021 Nov 23;39:107598. doi: 10.1016/j.dib.2021.107598. eCollection 2021 Dec.
Experiments employing the Phenotype Mammalian Microarray (PM-M) technology were performed on lymphoblastoid cell lines (LCLs) from individuals with autism spectrum disorder (ASD) and age-matched controls. We used the custom-made PM-M plate designed to assess differential utilization of the amino acid tryptophan. Multiple parameters such as the sample size, incubation time, and cell concentration have been tested, leading to optimized protocols and minimized background noise by variable selection while controlling for false discoveries. The assay generated data based on the production of nicotinamide adenine dinucleotide (NADH) in the presence of different compounds containing tryptophan and showed clear differences between ASD and control samples.
采用表型哺乳动物微阵列（PM-M）技术对来自自闭症谱系障碍（ASD）个体和年龄匹配对照的淋巴母细胞系（LCL）进行了实验。我们使用了定制的PM-M板来评估色氨酸的差异利用情况。对样本量、孵育时间和细胞浓度等多个参数进行了测试，通过变量选择优化了实验方案，并在控制错误发现的同时将背景噪声降至最低。该测定基于在含有色氨酸的不同化合物存在下烟酰胺腺嘌呤二核苷酸（NADH）的产生生成数据，显示ASD样本和对照样本之间存在明显差异。
