Department of Diagnostic and Health Sciences, University of Tennessee Health Science Center, Memphis, TN, USA.
Pathology Department, Methodist University Hospital, Memphis, TN, USA.
J Histotechnol. 2022 Jun;45(2):77-84. doi: 10.1080/01478885.2021.1996125. Epub 2021 Dec 8.
Analysis of surgical pathology specimens by histological techniques including immunohistochemistry (IHC) assays is a mainstay of disease diagnosis in humans. Neutral buffered formalin (NBF) is currently the primary fixative used, but its use is not without risks due to toxicity and carcinogenicity. Several glyoxal-based fixatives have been commercially produced, are considered safer alternatives to NBF, and produce histochemical staining results comparable to that of tissues fixed in NBF. However, previous studies evaluating IHC assay results in tissues fixed in NBF and glyoxal solutions have indicated mixed results. This study demonstrated that while tissues fixed in NBF were slightly superior to tissues fixed in glyoxal solutions among the 34 antibodies assayed with IHC, all fixative solutions produced results compatible for use in an anatomic pathology laboratory.
通过包括免疫组织化学（IHC）检测在内的组织学技术对手术病理标本进行分析是人类疾病诊断的主要方法。目前，中性缓冲福尔马林（NBF）是主要的固定剂，但由于其毒性和致癌性，使用它并非没有风险。已经商业化生产了几种基于乙二醛的固定剂，被认为是 NBF 的更安全替代品，并且产生的组织化学染色结果与 NBF 固定的组织相似。然而，以前评估使用 NBF 和乙二醛溶液固定的组织的 IHC 检测结果的研究表明结果不一。这项研究表明，在使用 IHC 检测的 34 种抗体中，虽然 NBF 固定的组织略优于乙二醛溶液固定的组织，但所有固定剂溶液都产生了适合在解剖病理学实验室使用的结果。
