Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA), 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan.
Medical SV, Kaneka Corporation, 1-8 Miyamaemachi, Takasago-cho, Takasago, Hyogo 676-8688, Japan.
J Med Microbiol. 2021 Dec;70(12). doi: 10.1099/jmm.0.001464.
Non-tuberculosis mycobacterium infections are increasing worldwide, including those caused by rapidly growing mycobacteria (RGM). The identification of the aetiological agent in the context of infections is essential for the adoption of an adequate therapeutic approach. However, the methods for the rapid distinction of different RGM species are less than optimal. To develop a nucleic acid chromatography kit to identify clinically common RGM. We tried to develop a nucleic acid chromatography kit designed to detect four RGM species (including three subspecies) i.e. subsp. , subsp. (detected as ) subsp. , , and . The amplified target genes for each species/subspecies using multiplex PCR were analysed using a nucleic acid chromatography assay. Among the 159 mycobacterial type strains and 70 RGM clinical isolates tested, the developed assay correctly identified all relevant RGM without any cross-reactivity or false-negatives. The limits of detection for each species were approximately 0.2 pg µl. The rapid and simple nucleic acid chromatography method developed here, which does not involve heat denaturation, may contribute to the rapid identification and treatment of RGM infections.
非结核分枝杆菌感染在全球范围内呈上升趋势，包括由快速生长分枝杆菌（RGM）引起的感染。在感染背景下鉴定病原体对于采取适当的治疗方法至关重要。然而，快速区分不同 RGM 物种的方法并不完善。为了开发一种用于鉴定临床常见 RGM 的核酸色谱试剂盒。我们试图开发一种核酸色谱试剂盒，用于检测四种 RGM 物种（包括三个亚种），即 subsp. 、 subsp. （检测为 subsp. ）、 subsp. 、 subsp. 和 subsp. 。使用多重 PCR 分析针对每个物种/亚种扩增的靶基因，并使用核酸色谱分析进行分析。在所测试的 159 株分枝杆菌标准菌株和 70 株 RGM 临床分离株中，开发的检测方法正确鉴定了所有相关的 RGM，没有任何交叉反应或假阴性。每个物种的检测限约为 0.2pgµl。这里开发的快速、简单的核酸色谱方法不涉及热变性，可能有助于快速鉴定和治疗 RGM 感染。
