Adekambi Toidi, Sassi Mohamed, van Ingen Jakko, Drancourt Michel
Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, USA.
University of Rennes 1, Inserm U835 Biochimie Pharmaceutique, Rennes, France.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2726-2730. doi: 10.1099/ijsem.0.002011. Epub 2017 Aug 18.
TheMycobacterium abscessus complex is a group of rapidly growing, multiresistant mycobacteria previously divided into three species. Proposal for the union of Mycobacterium bolletii and Mycobacterium massiliense into one subspecies, so-called M. abscessus subsp. massiliense, created much confusion about the routine identification and reporting of M. abscessus clinical isolates for clinicians. Results derived from multigene sequencing unambiguously supported the reinstatement of M. massiliense and M. bolletii as species, culminating in the presence of erm(41)-encoded macrolide resistance in M. bolletii. Present genome-based analysis unambiguously supports the reinstatement of M. massiliense and M. bolletii as species after the average nucleotide identity values of 96.7 % for M. abscessus versus M. bolletii, and 96.4 % for M. abscessus versus M. massiliense, and the 96.6 % identity between M. bolletii and M. massiliense was put into the perspective of a larger, 28-species analysis. Accordingly, DNA-DNA hybridization values predicted by the complete rpoB gene sequencing analysis were between 68.7 and 72.3 % in this complex. These genomic data as well as the phenotypic characteristics prompted us to propose to reinstate the previously known M. massiliense and M. bolletii into two distinct species among the M. abscessus complex.
脓肿分枝杆菌复合群是一组快速生长、具有多重耐药性的分枝杆菌，以前分为三个种。将博列特分枝杆菌和马赛分枝杆菌合并为一个亚种，即所谓的脓肿分枝杆菌马赛亚种，这给临床医生对脓肿分枝杆菌临床分离株的常规鉴定和报告造成了很大混乱。多基因测序结果明确支持将马赛分枝杆菌和博列特分枝杆菌恢复为独立的种，最终发现博列特分枝杆菌存在erm(41)编码的大环内酯耐药性。目前基于基因组的分析明确支持将马赛分枝杆菌和博列特分枝杆菌恢复为独立的种，因为脓肿分枝杆菌与博列特分枝杆菌的平均核苷酸同一性值为96.7%，脓肿分枝杆菌与马赛分枝杆菌的平均核苷酸同一性值为96.4%，并且在对28个种的更大规模分析背景下，博列特分枝杆菌与马赛分枝杆菌之间的同一性为96.6%。因此，通过完整rpoB基因测序分析预测的该复合群中的DNA-DNA杂交值在68.7%至72.3%之间。这些基因组数据以及表型特征促使我们提议将先前已知的马赛分枝杆菌和博列特分枝杆菌恢复为脓肿分枝杆菌复合群中的两个不同的种。
