Cornaglia-Ferraris P, Ponzoni M
Lymphokine Res. 1985 Summer;4(3):251-63.
The supernatant of TE+ lymphocytes isolated by density gradient from a lympho-epithelial thymoma contained a biochemical variant of T cell growth factor (IL-2). This protein showed two peaks with an apparent molecular weight of 18 and 28 kd by Sephadex G 100 chromatography. Further purification was achieved by DEAE Sephacel and CM Sepharose ion-exchange chromatography. By isoelectric focusing the isoelectric point was found to differ from that of normal. IL-2. Based on these results, the IL-2 variant appears to be a new kind of lymphokine distinguishable from normal IL-2 and similar to a TCGF (L-TCGF peak II) purified from the malignant T cell clone HUT 102.
从淋巴细胞上皮性胸腺瘤中通过密度梯度分离得到的TE + 淋巴细胞的上清液中含有T细胞生长因子（白细胞介素-2）的一种生化变体。通过葡聚糖凝胶G 100层析，这种蛋白质呈现出两个峰，表观分子量分别为18和28千道尔顿。通过DEAE 琼脂糖凝胶和CM 琼脂糖凝胶离子交换层析实现了进一步纯化。通过等电聚焦发现其等电点与正常白细胞介素-2不同。基于这些结果，白细胞介素-2变体似乎是一种新型淋巴因子，有别于正常白细胞介素-2，类似于从恶性T细胞克隆HUT 102中纯化得到的一种TCGF（L-TCGF峰II）。
