Department of Prosthodontics, University Medical Center Göttingen, Göttingen, Germany.
Institute for Organic and Biomolecular Chemistry, University of Göttingen, Göttingen, Germany.
Eur J Oral Sci. 2021 Dec;129(6):e12826. doi: 10.1111/eos.12826. Epub 2021 Dec 8.
This study aimed to investigate the release of common monomers from two conventional and two bisphenol A (BPA)-free temporary crown and bridge materials. Cylindrical samples of all materials were prepared (N = 90; five samples for each material and cycle of analysis). All samples were immersed in high-performance liquid chromatography (HPLC)-grade water and incubated for 1 h, 12 h, 24 h, and 7 days in an incubation shaker at 37°C and 112 rpm. Extraction was performed in accordance with ISO 10993-12. Eluted monomers were detected and quantified by HPLC coupled with ultraviolet-visible spectroscopy and mass spectrometry (HPLC-UV/Vis-MS). Analysis of BPA was performed by HPLC coupled with ultraviolet-visible spectroscopy (HPLC-UV/Vis) and positive results were verified by HPLC-tandem mass spectrometry (HPLC-MS/MS). Neither bisphenol A-glycidyl methacrylate (Bis-GMA) nor BPA was quantifiable in any of the crown and bridge samples investigated in the present study. However, all samples contained triethylene glycol dimethacrylate (TEGDMA) and/or urethane dimethacrylate (UDMA) after 24 h of incubation. Statistical analysis showed that significantly more UDMA was released from the BPA-free materials than from the conventional materials. All concentrations of UDMA measured were below the effective cytotoxic concentrations previously reported. However, for a few materials, especially BPA-free temporary crown and bridge materials, the levels of UDMA were above previously reported potentially harmful concentrations for local cells. As BPA-free materials were introduced as being more biocompatible than materials containing BPA, substitution of Bis-GMA with UDMA should be further investigated.
本研究旨在探讨两种传统型和两种双酚 A（BPA）自由型临时冠桥材料常见单体的释放情况。所有材料均制备圆柱形样本（N=90；每种材料和分析周期各五个样本）。所有样本均浸入高效液相色谱（HPLC）级水中，并在 37°C 和 112 rpm 的孵育摇床中孵育 1 h、12 h、24 h 和 7 天。根据 ISO 10993-12 进行萃取。洗脱单体通过高效液相色谱-紫外可见分光光度法和质谱联用（HPLC-UV/Vis-MS）进行检测和定量分析。通过高效液相色谱-紫外可见分光光度法（HPLC-UV/Vis）进行 BPA 分析，阳性结果通过高效液相色谱-串联质谱法（HPLC-MS/MS）验证。在本研究中，调查的所有冠桥样本中均未检测到或定量到双酚 A-缩水甘油甲基丙烯酸酯（Bis-GMA）或 BPA。然而，在孵育 24 h 后，所有样本均含有三乙二醇二甲基丙烯酸酯（TEGDMA）和/或尿烷二甲基丙烯酸酯（UDMA）。统计分析表明，BPA 自由型材料释放的 UDMA 明显多于传统型材料。测量的所有 UDMA 浓度均低于先前报道的有效细胞毒性浓度。然而，对于少数材料，尤其是 BPA 自由型临时冠桥材料，其 UDMA 水平高于先前报道的对局部细胞有潜在危害的浓度。由于 BPA 自由型材料被认为比含 BPA 的材料更具生物相容性，因此应进一步研究用 UDMA 替代 Bis-GMA。
