Department of Biophysics, Faculty of Science, Cairo University, Cairo, Egypt.
Department of Entomology, Faculty of Science, Cairo University, Cairo, Egypt.
Electromagn Biol Med. 2022 Jan 2;41(1):80-92. doi: 10.1080/15368378.2021.2012787. Epub 2021 Dec 8.
Electrical Pulsed Field (PEF), of pulse duration in 4 milliseconds, effect on mosquito larvae () as aquatic insects is assessed in this work. Mosquito larvae classes have been treated with electric field power values (66.66, 83.33, 100, 116.66 V/cm) with separate pulse number (60) and other classes of various pulse numbers have been treated (20, 40, 60, 80) with power of the electrical field 100 V/cm. The findings revealed that positively significant of increase of the applied electrical field strength or increase of the number of pulses. The rise in both cases leads to an increase in the mortality of 25%, 50%, and 75% of the mosquito larvae ( < .05). The impact was calculated with the bioassay system on mosque larvae, SDS-PAGE for whole body proteins, enzyme analysis and ultrastructural examination using TEM. The current study reveals that a low pulsed electric field can cause mosquito larvae genotoxic, changes in the insect's body proteins, which may affect the insect's ability to live. The increase in pulsed electric field parameters also activates oxidative stress in the insect cell by disrupting its secretion of enzymes that could affect the mosquito's capabilities in the future.
本研究评估了脉冲持续时间为 4 毫秒的电脉冲场（PEF）对水生昆虫蚊子幼虫的影响。对蚊子幼虫进行了电场功率值（66.66、83.33、100、116.66 V/cm）分别为 60 个脉冲和其他各种脉冲数（20、40、60、80）的电场功率为 100 V/cm 的处理。结果表明，应用电场强度或脉冲数的增加均呈正显著增加。在这两种情况下，死亡率均上升了 25%、50%和 75%（<0.05）。利用生物测定系统对蚊子幼虫进行了冲击评估，对全虫体蛋白进行了 SDS-PAGE 分析，对酶进行了分析，并使用 TEM 进行了超微结构检查。本研究表明，低强度脉冲电场可导致蚊子幼虫产生遗传毒性，改变昆虫的体内蛋白质，从而可能影响昆虫的生存能力。脉冲电场参数的增加也会通过破坏昆虫细胞的酶分泌来激活氧化应激，这可能会影响蚊子未来的能力。
