Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China,
Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Acta Haematol. 2022;145(2):176-183. doi: 10.1159/000520446. Epub 2021 Dec 8.
Circular RNAs (circRNAs) are a novel class of RNAs which occupy gene expression at the transcriptional or post-transcriptional level, involve in many physiological processes, and participate in many diseases, especially in cancer. Our previous study showed 1 altered circRNA named circ-anaphase promoting complex subunit 7 (ANAPC7) that was upregulated in acute myeloid leukemia (AML). To further clear the expression and clinical significance of circ-ANAPC7, we enlarged the sample size and illuminated the diagnostic and monitoring value of circ-ANAPC7 in AML.
Real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was supposed to confirm the expression of circ-ANAPC7 of AML patients. We assessed the correlation of circ-ANAPC7 and clinical variables using the Spearman correlation test. The receiver operating characteristic (ROC) curve was carried out to evaluate the diagnostic value.
Circ-ANAPC7 was first found to be upregulated in AML, and its expression was correlated to white blood cell counts in peripheral blood and blast percentage in bone marrow. ROC curve analysis revealed that circ-ANAPC7 has a significant value of auxiliary AML diagnosis (area under the curve = 0.915, p < 0.001). Furthermore, the expression level of circ-ANAPC7 was changed accompanied with disease condition transformation.
Circ-ANAPC7 was upregulated in newly diagnosed and relapsed AML. It may serve as potential biomarkers for AML patient's diagnosis and monitoring.
环状 RNA（circRNAs）是一类新型的 RNA，可在转录或转录后水平上占据基因表达，参与许多生理过程，并参与许多疾病，尤其是癌症。我们之前的研究表明，在急性髓系白血病（AML）中，一种名为环状有丝分裂促进复合物亚基 7（ANAPC7）的circRNA 发生改变，并且上调。为了进一步阐明 circ-ANAPC7 的表达和临床意义，我们扩大了样本量，并阐明了 circ-ANAPC7 在 AML 中的诊断和监测价值。
实时定量逆转录聚合酶链反应（RT-qPCR）用于确认 AML 患者 circ-ANAPC7 的表达。我们使用 Spearman 相关检验评估 circ-ANAPC7 与临床变量之间的相关性。进行接收者操作特征（ROC）曲线分析以评估诊断价值。
首先发现 circ-ANAPC7 在 AML 中上调，其表达与外周血白细胞计数和骨髓中原始细胞百分比相关。ROC 曲线分析表明，circ-ANAPC7 对辅助 AML 诊断具有重要价值（曲线下面积=0.915，p<0.001）。此外，circ-ANAPC7 的表达水平随疾病状况的变化而变化。
新诊断和复发的 AML 中 circ-ANAPC7 上调。它可能成为 AML 患者诊断和监测的潜在生物标志物。
