Chen Hongli, Liu Tian, Liu Jing, Feng Yuandong, Wang Baiyan, Wang Jianli, Bai Ju, Zhao Wanhong, Shen Ying, Wang Xiaman, Yang Juan, Ji Yuqiang, He Aili, Yang Yun
Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Department of Cell Biology and Genetics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, China.
Cell Physiol Biochem. 2018;47(5):1998-2007. doi: 10.1159/000491468. Epub 2018 Jul 3.
BACKGROUND/AIMS: Circular RNAs (circRNAs) are a family of novel non-coding RNAs associated with various diseases, especially cancer. Recent studies have demonstrated that circRNAs participate in pathogenesis mainly by acting as microRNA (miRNA) sponges. The expression profile of circRNAs in acute myeloid leukemia (AML) has rarely been reported.
Profiles of circRNAs were analyzed using an Arraystar human circRNA microarray with 5 bone marrow samples from patients with newly diagnosed AML and 5 from patients with iron-deficiency anemia. Quantitative reverse transcription PCR was used to validate the expression pattern of circRNAs. Furthermore, circRNA-miRNA network, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were applied.
CircRNA microarray analysis revealed that 698 circRNAs were differentially expressed in AML patients, with 282 circRNAs found to be upregulated and 416 to be downregulated. Quantitative reverse transcription PCR showed that circ-ANAPC7 was significantly upregulated in AML. Bioinformatics analysis predicted that circ-ANAPC7 acts as a sponge for the miR-181 family, KEGG analysis revealed that it is associated with cancer-related pathways, and GO analysis indicated that most of its target genes are involved in biological processes.
These findings show that circ-ANAPC7 is a promising biomarker for AML, and that it might participate in AML pathogenesis by acting as a sponge for the miR-181 family.
背景/目的：环状RNA（circRNAs）是一类新型非编码RNA，与多种疾病尤其是癌症相关。最近的研究表明，circRNAs主要通过充当微小RNA（miRNA）海绵参与发病机制。急性髓系白血病（AML）中circRNAs的表达谱鲜有报道。
使用Arraystar人类circRNA微阵列分析5例新诊断AML患者和5例缺铁性贫血患者的骨髓样本中的circRNAs谱。采用定量逆转录PCR验证circRNAs的表达模式。此外，还应用了circRNA-miRNA网络、基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路分析。
circRNA微阵列分析显示，698个circRNAs在AML患者中差异表达，其中282个circRNAs上调，416个下调。定量逆转录PCR显示circ-ANAPC7在AML中显著上调。生物信息学分析预测circ-ANAPC7充当miR-181家族的海绵，KEGG分析显示它与癌症相关通路有关，GO分析表明其大多数靶基因参与生物学过程。
这些发现表明circ-ANAPC7是AML有前景的生物标志物，并且它可能通过充当miR-181家族的海绵参与AML发病机制。
