College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
Plant Dis. 2022 Jun;106(6):1730-1735. doi: 10.1094/PDIS-11-21-2430-RE. Epub 2022 May 5.
Clubroot caused by is a serious threat to cruciferous crops around the world. The resting spores of are a primary source of infection and can survive in soil for many years. Detection of resting spores in soil is essential for forecasting clubroot prevalence. Detection of has been relying on plant bioassays or PCR-based methods. The loop-mediated isothermal DNA amplification (LAMP) is a promising approach for microorganism detection with the advantage of high sensitivity, accuracy, and convenience in viewing. In this study, we developed a LAMP assay for detection of in soil, roots, and seeds. This method can detect at a minimal amount of 1 fg of plasmid DNA or 10 resting spores in the soil. Compared with conventional PCR, the LAMP was more sensitive in detection of at the lower levels in soil samples. In conclusion, we elaborated a sensitive, accurate, and easy-to-use LAMP assay to detect , which will facilitate sustainable clubroot management and planning.
由 引起的根肿病是全世界十字花科作物的严重威胁。 休眠孢子是主要的感染源，能够在土壤中存活多年。检测土壤中的休眠孢子对于预测根肿病的流行至关重要。目前，对 的检测一直依赖于植物生物测定或基于 PCR 的方法。环介导等温 DNA 扩增 (LAMP) 是一种很有前途的微生物检测方法，具有高灵敏度、准确性和便于观察的优点。在本研究中，我们开发了一种用于检测土壤、根和种子中 的 LAMP 检测方法。该方法可以检测到土壤中少至 1 fg 的质粒 DNA 或 10 个休眠孢子。与传统 PCR 相比，该 LAMP 方法在土壤样本中较低水平检测 时更灵敏。总之，我们详细阐述了一种敏感、准确和易于使用的 LAMP 检测方法来检测 ，这将有助于可持续的根肿病管理和规划。
