Li Hong-Miao, Huang Qian, Tang Fen, Zhang Tian-Ping
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, Hefei, 230032, Anhui, People's Republic of China.
Anhui Chest Hospital (Anhui Provincial TB Institute), Hefei, 230022, Anhui, People's Republic of China.
Int J Gen Med. 2021 Dec 1;14:9203-9209. doi: 10.2147/IJGM.S339194. eCollection 2021.
Reactive oxygen species (ROS) generated by NADPH oxidase has a pivotal role in the nonspecific innate immune response to invading microorganisms including (MTB). NCF2 and NOX2 were considered as important functional subunits of NADPH oxidase complex; hence, this study aimed to evaluate the NCF2, NOX2 mRNA expressions in PBMC of pulmonary tuberculosis (PTB) patients.
A total of 79 PTB patients and 73 controls were included in our study. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to measure the NCF2, NOX2 mRNA levels, and receiver operating characteristic (ROC) curve analysis was performed to assess the diagnostic value of NCF2, NOX2 in PTB patients.
When compared to controls, the NCF2, NOX2 mRNA levels were significantly increased in PBMC from PTB patients ( < 0.001). However, the NCF2, NOX2 mRNA levels were not associated with major clinical and laboratory data of PTB patients. Area under curve (AUC) of ROC curve analysis for NCF2 and NOX2 were 0.686 (95% CI: 0.601, 0.770) and 0.705 (95% CI: 0.623, 0.787), respectively.
Altered NCF2, NOX2 mRNA levels in PTB patients implied that these genes might play roles in PTB, and their expression levels might be potential biomarkers for the diagnosis of PTB.
烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶产生的活性氧（ROS）在对包括结核分枝杆菌（MTB）在内的入侵微生物的非特异性固有免疫反应中起关键作用。NCF2和NOX2被认为是NADPH氧化酶复合物的重要功能亚基；因此，本研究旨在评估肺结核（PTB）患者外周血单个核细胞（PBMC）中NCF2、NOX2 mRNA的表达。
本研究共纳入79例PTB患者和73例对照。采用定量实时逆转录聚合酶链反应（qRT-PCR）检测NCF2、NOX2 mRNA水平，并进行受试者工作特征（ROC）曲线分析，以评估NCF2、NOX2在PTB患者中的诊断价值。
与对照组相比，PTB患者PBMC中NCF2、NOX2 mRNA水平显著升高（<0.001）。然而，NCF2、NOX2 mRNA水平与PTB患者的主要临床和实验室数据无关。NCF2和NOX2的ROC曲线分析曲线下面积（AUC）分别为0.686（95%CI：0.601，0.770）和0.705（95%CI：0.623，0.787）。
PTB患者NCF2、NOX2 mRNA水平的改变表明这些基因可能在PTB中起作用，其表达水平可能是PTB诊断的潜在生物标志物。
