Balkrishna Acharya, Lochab Savita, Joshi Monali, Srivastava Jyotish, Varshney Anurag
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar, 249405, Uttarakhand, India.
Department of Allied and Applied Sciences, University of Patanjali, Patanjali Yog Peeth, Haridwar, 249405, Uttarakhand, India.
J Exp Pharmacol. 2021 Dec 2;13:937-955. doi: 10.2147/JEP.S328864. eCollection 2021.
Divya-Herbal-Peya (DHP) is a plant-based decoction containing fourteen herbs in precise quantities; usually prescribed by the practitioners in Ayurveda to alleviate stress and minimize the exasperating symptoms of recurring infections. Our study aims to provide an experimental validation to the immunomodulatory properties of DHP.
Physico-chemical analysis of DHP was performed to evaluate the presence of secondary metabolites. The phytochemicals were then identified and quantitated through HPTLC, UHPLC, and GC-MS techniques. To address the scientific rationale behind DHP, lipopolysaccharide (LPS) was intraperitoneally injected in adult zebrafish to develop inflammatory response. Following LPS-induction, abnormality in locomotory behaviour was determined by evaluating the relative swim velocity and the rate of turning in experimental zebrafish. Pathophysiological effects were determined through opercular frequency, behavioural fever, and caudal fin damage. LPS-mediated inflammation was measured of pro-inflammatory cytokines, TNFα, IL-6, and IL-1β expression in the serum of study animals, by RT-PCR.
Our study phytochemically characterized and ascertained the presence of glycyrrhizin, rosmarinic acid, gingerol, cinnamic acid, protocatechuic acid, gallic acid, ellagic acid, piperine and cinnamaldehyde in DHP decoction. LPS induced aberrant locomotory patterns, behavioural fever and caudal fin damage in zebrafish. A significant increase in gene expression levels of pro-inflammatory cytokines, TNFα, IL-6, and IL-1β was also determined. However, these locomotory deviations and behavioural fever were negligible in zebrafish groups pre-administered either with DHP in a dose dependent manner or dexamethasone (DEX). The altered opercular rate, caudal fin damage and elevated transcription levels of pro-inflammatory genes upon LPS-induction were averted in groups pre-treated with DHP and DEX.
DHP prophylactically prevented the LPS-induced abnormal behaviour and inflammation-related pathophysiology in zebrafish. Immunomodulatory properties of DHP may not have therapeutic intervention, but do confer nutraceutical health benefits against mild infections.
Divya-Herbal-Peya（DHP）是一种植物性煎剂，含有精确数量的十四种草药；阿育吠陀从业者通常会开这种药来缓解压力，并将反复感染的恼人症状降至最低。我们的研究旨在为DHP的免疫调节特性提供实验验证。
对DHP进行物理化学分析，以评估次生代谢产物的存在。然后通过高效薄层色谱法（HPTLC）、超高效液相色谱法（UHPLC）和气相色谱-质谱联用技术（GC-MS）对植物化学物质进行鉴定和定量。为了探究DHP背后的科学原理，将脂多糖（LPS）腹腔注射到成年斑马鱼体内以引发炎症反应。在LPS诱导后，通过评估实验斑马鱼的相对游泳速度和转弯速率来确定运动行为异常。通过鳃盖运动频率、行为性发热和尾鳍损伤来确定病理生理效应。通过逆转录聚合酶链反应（RT-PCR）测量研究动物血清中促炎细胞因子肿瘤坏死因子α（TNFα）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）的表达，以检测LPS介导的炎症。
我们的研究对DHP进行了植物化学特征分析，并确定了DHP煎剂中存在甘草甜素、迷迭香酸、姜辣素、肉桂酸、原儿茶酸、没食子酸、鞣花酸、胡椒碱和肉桂醛。LPS在斑马鱼中诱导了异常的运动模式、行为性发热和尾鳍损伤。还确定促炎细胞因子TNFα、IL-6和IL-1β的基因表达水平显著增加。然而，在以剂量依赖性方式预先给予DHP或地塞米松（DEX）的斑马鱼组中，这些运动偏差和行为性发热可以忽略不计。在预先用DHP和DEX处理的组中，LPS诱导后的鳃盖运动速率改变、尾鳍损伤和促炎基因转录水平升高得到了避免。
DHP预防性地预防了LPS诱导的斑马鱼异常行为和与炎症相关的病理生理。DHP的免疫调节特性可能没有治疗干预作用，但确实能为抵御轻度感染带来营养保健益处。
