Ma Haijie, Li Lei, Gai Yunpeng, Zhang Xiaoyan, Chen Yanan, Zhuo Xiaokang, Cao Yingzi, Jiao Chen, Gmitter Fred G, Li Hongye
Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A&F University, Hangzhou, China.
Key Lab of Molecular Biology of Crop Pathogens and Insects, Ministry of Agriculture, Institute of Biotechnology, Zhejiang University, Hangzhou, China.
Front Microbiol. 2021 Nov 22;12:783633. doi: 10.3389/fmicb.2021.783633. eCollection 2021.
Histone acetylation, which is critical for transcriptional regulation and various biological processes in eukaryotes, is a reversible dynamic process regulated by HATs and HDACs. This study determined the function of 6 histone acetyltransferases (HATs) (, , , , , ) and 6 histone deacetylases (HDACs) (, , , , , ) in the phytopathogenic fungus by analyzing targeted gene deletion mutants. Our data provide evidence that HATs and HDACs are both required for mycelium growth, cell development and pathogenicity as many gene deletion mutants (Δ, Δ, Δ, Δ, Δ, Δ, and Δ) displayed reduced growth, conidiation or virulence at varying degrees. In addition, HATs and HDACs are involved in the resistance to multiple stresses such as oxidative stress (, , , , ), osmotic stress (, , , ), cell wall-targeting agents (, , ), and fungicide (, ). Δ, Δ, and Δ displayed severe growth defects on sole carbon source medium suggesting a vital role of HATs and HDACs in carbon source utilization. More SNPs were generated in Δ in comparison to wild-type when they were exposed to ultraviolet ray. Moreover, Δ, Δ, and Δ showed severe defects in resistance to DNA-damaging agents, indicating the critical role of HATs and HDACs in DNA damage repair. These phenotypes correlated well with the differentially expressed genes in Δ and Δ that are essential for carbon sources metabolism, DNA damage repair, ROS detoxification, and asexual development. Furthermore, is required for the acetylation of H3K4. Overall, our study provides genetic evidence to define the central role of HATs and HDACs in the pathological and biological functions of .
组蛋白乙酰化对于真核生物的转录调控和各种生物学过程至关重要，是一个由组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）调控的可逆动态过程。本研究通过分析靶向基因缺失突变体，确定了6种组蛋白乙酰转移酶（HATs）（ 、 、 、 、 、 ）和6种组蛋白去乙酰化酶（HDACs）（ 、 、 、 、 、 ）在植物病原真菌 中的功能。我们的数据表明，HATs和HDACs对于菌丝体生长、细胞发育和致病性都是必需的，因为许多基因缺失突变体（Δ 、Δ 、Δ 、Δ 、Δ 、Δ 和Δ ）均表现出不同程度的生长、分生孢子形成或毒力降低。此外，HATs和HDACs参与对多种胁迫的抗性，如氧化胁迫（ 、 、 、 、 ）、渗透胁迫（ 、 、 ）、细胞壁靶向剂（ 、 、 ）和杀菌剂（ 、 ）。Δ 、Δ 和Δ 在单一碳源培养基上表现出严重的生长缺陷，表明HATs和HDACs在碳源利用中起着至关重要的作用。与野生型相比，Δ 在暴露于紫外线时产生了更多的单核苷酸多态性（SNPs）。此外，Δ 、Δ 和Δ 在对DNA损伤剂的抗性方面表现出严重缺陷，表明HATs和HDACs在DNA损伤修复中起关键作用。这些表型与Δ 和Δ 中差异表达的基因密切相关，这些基因对于碳源代谢、DNA损伤修复、活性氧解毒和无性发育至关重要。此外， 是H3K4乙酰化所必需的。总体而言，我们的研究提供了遗传学证据，以确定HATs和HDACs在 的病理和生物学功能中的核心作用。
