Hanazawa S, Ohmori Y, Amano S, Hirose K, Miyoshi T, Kumegawa M, Kitano S
FEBS Lett. 1986 Jul 28;203(2):279-84. doi: 10.1016/0014-5793(86)80758-x.
We examined the effects of human purified interleukin-1 (IL-1) on DNA synthesis, cell growth, and alkaline phosphatase activity in the osteoblastic cell line MC3T3-E1 under both preconfluent and confluent culture conditions. Addition of IL-1 to the cells markedly inhibited their DNA synthesis and growth over the range 1-10 U/ml. Such significant inhibitory effects were observed in cells cultivated in 1 or 5% fetal calf serum (FCS)-containing alpha modification Eagle's medium (alpha-MEM), but not in alpha-MEM containing 10% FCS. In contrast, alkaline phosphatase activity was enhanced significantly by IL-1 in the cell line cultivated in 1% FCS-containing alpha-MEM. These results demonstrate that human purified IL-1 is effective in inducing the differentiation of osteoblastic cell MC3T3-E1.
我们研究了人纯化白细胞介素-1（IL-1）在预汇合和汇合培养条件下对成骨细胞系MC3T3-E1中DNA合成、细胞生长和碱性磷酸酶活性的影响。向细胞中添加IL-1在1-10 U/ml范围内显著抑制其DNA合成和生长。在含有1%或5%胎牛血清（FCS）的α-改良伊格尔培养基（α-MEM）中培养的细胞中观察到这种显著的抑制作用，但在含有10%FCS的α-MEM中未观察到。相反，在含有1%FCS的α-MEM中培养的细胞系中，IL-1显著增强了碱性磷酸酶活性。这些结果表明，人纯化IL-1在诱导成骨细胞MC3T3-E1分化方面是有效的。
