Edmond H. Fischer Signal Transduction Laboratory, College of Life Sciences, Jilin University, Changchun, China.
Scientific Research Centre of China-Japan Union Hospital, Jilin University, Changchun, China.
Nanomedicine. 2022 Feb;40:102507. doi: 10.1016/j.nano.2021.102507. Epub 2021 Dec 6.
One of the most promising treatments for neurodegenerative diseases is the stem cell therapy; however, there are still some limitations in the treatment of Alzheimer's disease. In this study, superparamagnetic nanoparticles composed of magnetic FeO and polydopamine shells were used to label human umbilical cord mesenchymal stem cells (hUC-MSCs) in order to increase the targeting of hUC-MSCs. Our data suggested that FeO@PDA labeling increase the efficiency of hUC-MSCs entering the brain. Moreover, the water maze test showed that compared with hUC-MSCs only, FeO@PDA-labeled hUC-MSCs improved the cognitive ability of APP/PS1 transgenic mice more significantly. Other experimental data showed that the expression of essential proteins in the hippocampus, such as Aβ, synaptophysin, brain-derived neurotrophic factor, are affected by FeO@PDA coated-hUC-MSCs. The regulation of FeO@PDA coated-hUC-MSCs could improve the memory and cognitive ability of AD mice by excessive generation of neuroprotective factors, which might be considered a viable therapy to treat AD.
用于神经退行性疾病的最有前途的治疗方法之一是干细胞疗法；然而，阿尔茨海默病的治疗仍然存在一些局限性。在这项研究中，使用由磁性 FeO 和聚多巴胺壳组成的超顺磁纳米粒子来标记人脐带间充质干细胞（hUC-MSCs），以增加 hUC-MSCs 的靶向性。我们的数据表明，FeO@PDA 标记提高了 hUC-MSCs 进入大脑的效率。此外，水迷宫试验表明，与仅 hUC-MSCs 相比，FeO@PDA 标记的 hUC-MSCs 更显著地改善了 APP/PS1 转基因小鼠的认知能力。其他实验数据表明，海马中关键蛋白的表达，如 Aβ、突触小体蛋白、脑源性神经营养因子，受到 FeO@PDA 涂层-hUC-MSCs 的影响。FeO@PDA 涂层-hUC-MSCs 的调节可以通过过度产生神经保护因子来改善 AD 小鼠的记忆和认知能力，这可能被认为是治疗 AD 的一种可行疗法。
