Dahllöf G, Otteskog P, Modéer T
Scand J Dent Res. 1986 Jun;94(3):202-7. doi: 10.1111/j.1600-0722.1986.tb01754.x.
The effect of phenytoin and its major metabolite p-HPPH on concanavalin A (ConA) induced DNA-synthesis of human lymphocytes was studied in vitro. In lymphocyte cultures depleted of phagocytizing cells by iron treatment PHT and p-HPPH, in pharmacologic concentrations, caused a depression of the mitogen response measured as uptake of 3H-thymidine. In contrast, the presence of phagocytizing mononuclear cells during ConA-stimulation in the presence of PHT and p-HPPH caused a potentiation of ConA induced DNA-synthesis. These effects of phenytoin on immunocompetent cells may be of significance in the pathogenesis of the PHT-induced gingival overgrowth, since earlier we have reported the presence of large infiltrates of lymphocytes, mainly T-lymphocytes in gingival biopsies from clinically non-inflamed PHT-induced gingival overgrowth.
体外研究了苯妥英及其主要代谢产物对羟基苯妥英（p-HPPH）对刀豆蛋白A（ConA）诱导的人淋巴细胞DNA合成的影响。在用铁处理去除吞噬细胞的淋巴细胞培养物中，药理浓度的苯妥英（PHT）和p-HPPH导致以3H-胸腺嘧啶摄取量衡量的促有丝分裂原反应降低。相反，在PHT和p-HPPH存在的情况下，ConA刺激期间吞噬性单核细胞的存在导致ConA诱导的DNA合成增强。苯妥英对免疫活性细胞的这些作用可能在PHT诱导的牙龈过度生长的发病机制中具有重要意义，因为我们之前报道过，在临床上无炎症的PHT诱导的牙龈过度生长的牙龈活检中存在大量淋巴细胞浸润，主要是T淋巴细胞。
