Dahllöf G, Modèer T, Otteskog P, Sundqvist K G
Scand J Dent Res. 1985 Dec;93(6):507-12. doi: 10.1111/j.1600-0722.1985.tb01348.x.
The presence of mononuclear cells was studied in gingival biopsies from seven children exhibiting phenytoin(PHT)-induced gingival overgrowth, three children with gingivitis and a control group consisting of three children without clinical signs of inflammation. The mononuclear cells were detected using monoclonal antibodies defining functional T-lymphocyte subpopulations, B-lymphocytes and monocytes. Gingival biopsies from the individuals in the PHT-group showed a substantial number of mononuclear cells. The distribution of mononuclear cells in separate individuals were as follows: 69-95% OKT3 +/Leu4+ cells (T-lymphocytes), 50-64% OKT4 +/Leu3+ cells (T-helper phenotype) and 29-46% OKT8+ cells (T-suppressor/cytotoxic phenotype). None of the biopsies in the PHT-group contained more than a few scattered plasma cells. The vast majority of all mononuclear cells present in the biopsies reacted with OKIa1, a monoclonal antibody defining the HLA-DR framework. In contrast, biopsies from the control group and the gingivitis group contained few mononuclear cells, the majority of which were T-cells. This suggests that immunologic reactions mediated by T-cells may play a role in the pathogenesis of the PHT-induced lesion.
对7名表现出苯妥英（PHT）诱导的牙龈增生的儿童、3名患有牙龈炎的儿童以及由3名无炎症临床体征的儿童组成的对照组的牙龈活检组织中的单核细胞进行了研究。使用定义功能性T淋巴细胞亚群、B淋巴细胞和单核细胞的单克隆抗体检测单核细胞。PHT组个体的牙龈活检组织显示有大量单核细胞。单核细胞在不同个体中的分布如下：69 - 95%为OKT3 +/Leu4+细胞（T淋巴细胞），50 - 64%为OKT4 +/Leu3+细胞（T辅助表型），29 - 46%为OKT8+细胞（T抑制/细胞毒性表型）。PHT组的活检组织中均未发现超过少数散在的浆细胞。活检组织中存在的所有单核细胞绝大多数与OKIa1反应，OKIa1是一种定义HLA - DR框架的单克隆抗体。相比之下，对照组和牙龈炎组的活检组织中单核细胞很少，其中大多数是T细胞。这表明由T细胞介导的免疫反应可能在PHT诱导病变的发病机制中起作用。
