Department of Physics, Sogang University, Seoul, Korea.
Department of BioNanoScience Kavli Institute of Nanoscience of Delft University of Technology, Delft, The Netherlands.
Sci Rep. 2021 Dec 9;11(1):23721. doi: 10.1038/s41598-021-03148-4.
DNA cyclization assay together with single-molecule FRET was employed to monitor protein-mediated bending of a short dsDNA (~ 100 bp). This method provides a simple and easy way to monitor the structural change of DNA in real-time without necessitating prior knowledge of the molecular structures for the optimal dye-labeling. This assay was applied to study how Anabaena sensory rhodopsin transducer (ASRT) facilitates loop formation of DNA as a possible mechanism for gene regulation. The ASRT-induced DNA looping was maximized at 50 mM of Na, while Mg also played an essential role in the loop formation.
采用 DNA 环化分析与单分子Förster 共振能量转移（FRET）相结合的方法，实时监测蛋白介导的短双链 DNA（~100bp）弯曲。该方法无需预先了解最佳荧光染料标记的分子结构，提供了一种简单易用的监测 DNA 结构变化的方法。该方法用于研究蓝藻感应视紫红质转导蛋白（ASRT）如何促进 DNA 环化形成，这可能是一种基因调控的机制。ASRT 诱导的 DNA 环化在 50mM 的 Na 下达到最大值，而 Mg 也在环化形成中起关键作用。
