Schapiro Igor, Ruhman Sanford
Max Planck Institute for Chemical Energy Conversion, Stiftstr. 34-36, 45470 Mülheim an der Ruhr, Germany.
Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Biochim Biophys Acta. 2014 May;1837(5):589-97. doi: 10.1016/j.bbabio.2013.09.014. Epub 2013 Oct 5.
Light induced isomerization of the retinal chromophore activates biological function in all retinal protein (RP) driving processes such as ion-pumping, vertebrate vision and phototaxis in organisms as primitive as archea, or as complex as mammals. This process and its consecutive reactions have been the focus of experimental and theoretical research for decades. The aim of this review is to demonstrate how the experimental and theoretical research efforts can now be combined to reach a more comprehensive understanding of the excited state process on the molecular level. Using the Anabaena Sensory Rhodopsin as an example we will show how contemporary time-resolved spectroscopy and recently implemented excited state QM/MM methods consistently describe photochemistry in retinal proteins. This article is part of a Special Issue entitled: Retinal Proteins - You can teach an old dog new tricks.
视网膜发色团的光诱导异构化在所有视网膜蛋白（RP）驱动的过程中激活生物功能，这些过程包括离子泵浦、脊椎动物视觉以及在古细菌等原始生物或哺乳动物等复杂生物中的趋光性。几十年来，这一过程及其后续反应一直是实验和理论研究的重点。本综述的目的是展示如何将实验和理论研究成果结合起来，以便在分子水平上更全面地理解激发态过程。以鱼腥藻感光视紫红质为例，我们将展示当代时间分辨光谱学和最近实施的激发态量子力学/分子力学方法如何一致地描述视网膜蛋白中的光化学。本文是名为《视网膜蛋白——老狗也能学新招》特刊的一部分。
