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LOXL1-AS1 通过调节 miR-374b-5p/MMP14 轴与 TIAR 通讯调节胶质瘤中的血管生成拟态。

LOXL1-AS1 communicating with TIAR modulates vasculogenic mimicry in glioma via regulation of the miR-374b-5p/MMP14 axis.

机构信息

Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, China.

Liaoning Clinical Medical Research Center in Nervous System Disease, Shenyang, China.

出版信息

J Cell Mol Med. 2022 Jan;26(2):475-490. doi: 10.1111/jcmm.17106. Epub 2021 Dec 8.

DOI:10.1111/jcmm.17106
PMID:34890108
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8743654/
Abstract

At present, growing evidence indicates that long non-coding RNAs (lncRNAs) participate in the progression of glioma. The function of LOXL1-AS1 in vasculogenic mimicry (VM) in glioma remains unclear. First, the expressions of TIAR, the lncRNA LOXL1-AS1, miR-374b-5p and MMP14 were examined by qRT-PCR and Western blot in both, glioma tissues and glioma cell lines. Proliferation, migration, invasion and tube formation assays were conducted to evaluate the roles of TIAR, LOXL1-AS1, miR-374b-5p and MMP14 in malignant cellular behaviours in glioma cells. A nude mouse xenograft model and dual staining for CD34 and PAS were used to assess whether VM was affected by TIAR, LOXL1-AS1 or miR-374b-5p in vivo. In this study, low levels of TIAR and high levels of LOXL1-AS1 were found in glioma cells and tissues. TIAR downregulated the expression of LOXL1-AS1 by destabilizing it. LOXL1-AS1 acted like a miRNA sponge towards miR-374b-5p so that downregulation of the former greatly inhibited cell proliferation, migration, invasion and VM. Additionally, miR-374b-5p overexpression repressed malignant biological behaviours and VM in glioma by modifying MMP14. In summary, we demonstrated that TIAR combined with LOXL1-AS1 modulates VM in glioma via the miR-374b-5p/MMP14 axis, revealing novel targets for glioma therapy.

摘要

目前，越来越多的证据表明长非编码 RNA（lncRNA）参与了胶质瘤的进展。LOXL1-AS1 在胶质瘤中的血管生成拟态（VM）中的功能尚不清楚。首先，通过 qRT-PCR 和 Western blot 检测了胶质瘤组织和胶质瘤细胞系中 TIAR、lncRNA LOXL1-AS1、miR-374b-5p 和 MMP14 的表达。通过增殖、迁移、侵袭和管形成实验评估了 TIAR、LOXL1-AS1、miR-374b-5p 和 MMP14 在胶质瘤细胞恶性细胞行为中的作用。裸鼠异种移植模型和 CD34 和 PAS 双重染色用于评估 TIAR、LOXL1-AS1 或 miR-374b-5p 在体内对 VM 的影响。在本研究中，发现 TIAR 水平低，LOXL1-AS1 水平高在胶质瘤细胞和组织中。TIAR 通过使其不稳定而下调 LOXL1-AS1 的表达。LOXL1-AS1 充当 miR-374b-5p 的 miRNA 海绵，因此前者的下调极大地抑制了细胞增殖、迁移、侵袭和 VM。此外，miR-374b-5p 的过表达通过修饰 MMP14 抑制了胶质瘤中恶性生物学行为和 VM。总之，我们证明 TIAR 与 LOXL1-AS1 通过 miR-374b-5p/MMP14 轴调节胶质瘤中的 VM，为胶质瘤治疗提供了新的靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b032/8743654/ab5b450eebcb/JCMM-26-475-g003.jpg
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本文引用的文献

1
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Cancer Biomark. 2021;30(4):355-363. doi: 10.3233/CBM-190538.
2
MicroRNA-506-3p increases the response to PARP inhibitors and cisplatin by targeting EZH2/β-catenin in serous ovarian cancers.微小RNA-506-3p通过靶向浆液性卵巢癌中的EZH2/β-连环蛋白增加对PARP抑制剂和顺铂的反应。
Transl Oncol. 2021 Feb;14(2):100987. doi: 10.1016/j.tranon.2020.100987. Epub 2020 Dec 22.
3
Upregulation lnc-NEAT1 contributes to colorectal cancer progression through sponging miR-486-5p and activating NR4A1/Wnt/β-catenin pathway.
Immunotargets Ther. 2025 Mar 24;14:259-278. doi: 10.2147/ITT.S512768. eCollection 2025.
4
Identification of new subtypes of breast cancer based on vasculogenic mimicry related genes and a new model for predicting the prognosis of breast cancer.基于血管生成拟态相关基因的乳腺癌新亚型鉴定及乳腺癌预后预测新模型
Heliyon. 2024 Aug 20;10(17):e36565. doi: 10.1016/j.heliyon.2024.e36565. eCollection 2024 Sep 15.
5
Long Non-Coding H19 in Lymphocytes: Prognostic Value in Acute Ischemic Stroke Patients.淋巴细胞中的长链非编码H19：急性缺血性中风患者的预后价值
Pharmaceuticals (Basel). 2024 Jul 31;17(8):1008. doi: 10.3390/ph17081008.
6
A comprehensive review on lncRNA LOXL1-AS1: molecular mechanistic pathways of lncRNA LOXL1-AS1 in tumorigenicity of cancer cells.关于lncRNA LOXL1-AS1的全面综述：lncRNA LOXL1-AS1在癌细胞致瘤性中的分子机制途径
Front Oncol. 2024 Jul 29;14:1384342. doi: 10.3389/fonc.2024.1384342. eCollection 2024.
7
Involvement of long non-coding RNA LOXL1-AS1 in the tumourigenesis and development of malignant tumours: a narrative review.长链非编码RNA LOXL1-AS1在恶性肿瘤发生发展中的作用：一项叙述性综述
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8
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9
Potential role of lncRNA LOXL1-AS1 in human cancer development: a narrative review.长链非编码RNA LOXL1-AS1在人类癌症发展中的潜在作用：一篇叙述性综述
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10
Long non-coding RNA LOXL1-AS1: a potential biomarker and therapeutic target in human malignant tumors.长非编码 RNA LOXL1-AS1：人类恶性肿瘤的潜在生物标志物和治疗靶点。
Clin Exp Med. 2024 May 2;24(1):93. doi: 10.1007/s10238-024-01355-7.
上调长链非编码 RNA-NEAT1 通过海绵吸附 miR-486-5p 并激活 NR4A1/Wnt/β-连环蛋白通路促进结直肠癌的进展。
Cancer Biomark. 2021;30(3):309-319. doi: 10.3233/CBM-201733.
4
Long noncoding RNA ATB promotes ovarian cancer tumorigenesis by mediating histone H3 lysine 27 trimethylation through binding to EZH2.长链非编码 RNA ATB 通过与 EZH2 结合介导组蛋白 H3 赖氨酸 27 三甲基化促进卵巢癌细胞发生。
J Cell Mol Med. 2021 Jan;25(1):37-46. doi: 10.1111/jcmm.15329. Epub 2020 Dec 18.
5
LncRNA LINC00998 inhibits the malignant glioma phenotype via the CBX3-mediated c-Met/Akt/mTOR axis.长链非编码 RNA LINC00998 通过 CBX3 介导的 c-Met/Akt/mTOR 轴抑制恶性神经胶质瘤表型。
Cell Death Dis. 2020 Dec 2;11(12):1032. doi: 10.1038/s41419-020-03247-6.
6
RNA-binding proteins in human genetic disease.人类遗传疾病中的 RNA 结合蛋白。
Nat Rev Genet. 2021 Mar;22(3):185-198. doi: 10.1038/s41576-020-00302-y. Epub 2020 Nov 24.
7
LINC-PINT Suppresses the Aggressiveness of Thyroid Cancer by Downregulating miR-767-5p to Induce TET2 Expression.LINC-PINT通过下调miR-767-5p诱导TET2表达来抑制甲状腺癌的侵袭性。
Mol Ther Nucleic Acids. 2020 Jun 1;22:319-328. doi: 10.1016/j.omtn.2020.05.033. eCollection 2020 Dec 4.
8
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9
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10
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Int J Oncol. 2020 Dec;57(6):1293-1306. doi: 10.3892/ijo.2020.5134. Epub 2020 Oct 15.