Snow E C, Mond J J, Subbarao B
J Immunol. 1986 Sep 15;137(6):1793-6.
By using antigen-specific populations of B cells (TNP-ABC) we have demonstrated that the type-2 antigen TNP-Ficoll was capable of initiating B cell proliferation only in the presence of T cell-derived factors. Monoclonal-anti-Lyb-2.1 antibody acted synergistically with a T cell-derived supernatant, as well as with B cell-stimulating factor (BSF-1) to enhance the level of B cell expansion obtained in this in vitro system. This effect of anti-Lyb-2.1 mAB was observed at each day of the antigen-driven B cell expansion and was seen only with B cells purified from strains expressing the Lyb-2.1 allele. The epitope density of hapten on the Ficoll plays a critical role in this process, because Ficoll that is haptenated with low density of hapten was not found to be stimulatory. These results suggest that the Lyb-2 surface molecule influences the antigen-driven B cell growth that is stimulated by type 2 antigens and BSF-1.
通过使用B细胞的抗原特异性群体（TNP - ABC），我们已经证明，2型抗原TNP - 菲可（TNP - Ficoll）仅在存在T细胞衍生因子的情况下才能启动B细胞增殖。单克隆抗Lyb - 2.1抗体与T细胞衍生的上清液以及B细胞刺激因子（BSF - 1）协同作用，以提高在该体外系统中获得的B细胞扩增水平。抗Lyb - 2.1单克隆抗体的这种作用在抗原驱动的B细胞扩增的每一天都能观察到，并且仅在从表达Lyb - 2.1等位基因的品系中纯化的B细胞中可见。菲可上半抗原的表位密度在这个过程中起着关键作用，因为未发现用低密度半抗原进行半抗原化的菲可具有刺激作用。这些结果表明，Lyb - 2表面分子影响由2型抗原和BSF - 1刺激的抗原驱动的B细胞生长。
