College of Agricultural Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Shanxi Wanjiazhai Water Resources Co., Ltd., Xinzhou, Shanxi 030012, China.
Enzyme Microb Technol. 2022 Mar;154:109959. doi: 10.1016/j.enzmictec.2021.109959. Epub 2021 Dec 3.
In this paper a quick and disposable electrochemical biosensor based on bovine hemoglobin (BHb) is proposed for the determination of chlorpyrifos (CP). The bioelectrode (denoted as AuNPs/MoS-CS/BHb/SPE) has been designed by immobilizing BHb on the screen printed electrode (SPE) surface modified by gold nanoparticles (AuNPs) and Molybdenum disulfide (MoS) nanosheets - chitosan (CS) mixture (MoS-CS). Field emission scanning electron microscopy (FESEM) and electrochemical characterization demonstrate the successful grafting of AuNPs/MoS-CS/BHb/SPE. Detailed electrochemical impedance spectroscopy (EIS) analysis and cyclic voltammetry (CV) tests show that the proposed bioelectrode exhibits decent electrochemical properties, such as well conductivity and large specific surface area. In-depth electrochemical analysis reveals the redox reaction occurring on the surface of the bioelectrode and the mechanism that CP binds with BHb forming the thin film to inhibit the peak current. The proposed biosensor exhibits sensitive and stable responses for electrochemical assay of CP ranging from 0.004 μM to 28.52 μM, with a limit of detection (LOD) as 5.6 nM. The electrochemical biosensor passes the repeatability, reproducibility, stability and anti-interference ability test experiments with good performance. The biosensor also shows its credibility for detecting CP in real vegetable samples (Cabbage and Leek) with recovery (%) ranging from 87% to 109%. The proposed biosensor is of great potential application values for detecting CP and the study is of great reference value for the research and development of biosensors for quantitative detection of organophosphate pesticides (OPs).
本文提出了一种基于牛血红蛋白（BHb）的快速、一次性电化学生物传感器，用于测定毒死蜱（CP）。该生物电极（表示为 AuNPs/MoS-CS/BHb/SPE）通过将 BHb 固定在经金纳米粒子（AuNPs）和二硫化钼（MoS）纳米片-壳聚糖（CS）混合物（MoS-CS）修饰的丝网印刷电极（SPE）表面上而设计。场发射扫描电子显微镜（FESEM）和电化学表征证明了 AuNPs/MoS-CS/BHb/SPE 的成功接枝。详细的电化学阻抗谱（EIS）分析和循环伏安法（CV）测试表明，所提出的生物电极具有良好的导电性和较大的比表面积等优良的电化学性能。深入的电化学分析揭示了发生在生物电极表面的氧化还原反应以及 CP 与 BHb 结合形成薄膜以抑制峰电流的机制。所提出的生物传感器在 0.004 μM 至 28.52 μM 的范围内对 CP 的电化学分析表现出灵敏且稳定的响应，检测限（LOD）为 5.6 nM。电化学生物传感器通过具有良好性能的重复性、重现性、稳定性和抗干扰能力测试实验。该生物传感器还显示了其在真实蔬菜样品（白菜和韭菜）中检测 CP 的可信度，回收率（%）在 87%至 109%之间。该生物传感器在检测 CP 方面具有很大的潜在应用价值，该研究对开发用于定量检测有机磷农药（OPs）的生物传感器具有重要的参考价值。
