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[甲状旁腺组织的冷冻保存:一种简化方法的评估]

[Cryopreservation of parathyroid tissue: evaluation of a simplified method].

作者信息

Duff C, Largiadèr F

出版信息

Langenbecks Arch Chir. 1986;367(4):227-34. doi: 10.1007/BF01263403.

Abstract

A simplified, less expensive cryopreservation technique for parathyroid tissue was examined in animal study and on human tissue in vitro. More than 3/4 of all replantations in the rat were successful after cryopreservation. After replantation of one single gland only 1/3 of all animals attained normal parathyroid function. This fact shows the importance of the quantity of transplanted viable tissue. Viability of human parathyroid tissue after cryopreservation was 80-90%. The PTH-production of human parathyroid tissue in vitro after cryopreservation and the suppressibility in different calcium concentrations were similar to normal fresh tissue. All our results prove a good functioning of this simplified cryopreservation method.

摘要

在动物研究和人体组织体外实验中,对一种简化的、成本较低的甲状旁腺组织冷冻保存技术进行了研究。在大鼠中,超过四分之三的冷冻保存后再植手术获得成功。仅移植一个腺体后,所有动物中只有三分之一恢复了正常的甲状旁腺功能。这一事实表明了移植存活组织数量的重要性。人体甲状旁腺组织冷冻保存后的存活率为80%-90%。冷冻保存后的人体甲状旁腺组织在体外的甲状旁腺激素分泌以及在不同钙浓度下的可抑制性与正常新鲜组织相似。我们所有的结果都证明了这种简化冷冻保存方法的良好效果。

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