Mejia M L, Moncada M E, Ossa-Orozco C P
Annu Int Conf IEEE Eng Med Biol Soc. 2021 Nov;2021:1176-1180. doi: 10.1109/EMBC46164.2021.9629992.
In this work, electrospinning was used for the preparation of composite nanofibrous scaffold, of polyvinyl alcohol (PVA), silk fibroin (SF) extract of Bombyx mori cocoons and silver nanoparticles (Ag NPs), as a substrate for bone tissue engineering. The PVA pristine was prepared at a concentration of 10% wt. The composite nanofibers scaffolds of PVA was prepared with silk fibroin and silver nanoparticles, in relation of PVA: SF (90:10) (v/v) respectively. The formation and presence of AgNPs was confirmed by ultraviolet-visible spectroscopy (Uv-vis). The diameter distribution of the nanofibers was narrow by SEM using Image J software. The chemical composition was determined by FTIR spectra. The wettability was determined using water contact angle. The results showed the average nanofiber diameter of PVA10 pristine was 108.18 nm and to PVA10/SF/Ag NPs was 106.62 nm, no significant changes were noted in the mean diameter, but there were changes in its morphology. The average nanofiber diameter increase with the concentration of PVA at PVA15/SF/Ag NPs was 189.12 nm to PVA18/SF/Ag NPs was 224,23 nm. FTIR spectra indicated characteristic absorption peaks related to the chemical structure of PVA, fibroin and Ag NPs, it demonstrated good interactions between them, caused by strong intermolecular hydrogen bonds. The contact angle of the scaffolds PVA 10%wt decrease with the incorporation of fibroin and show hydrophilic characteristics. The achievements indicate the potential of the nanofibers of PVA15/SF/Ag NPs as a possible substitute for bone tissue engineering.Clinical Relevance-This establishes a possible substrate of PVA/SF/Ag NPs that exhibit desired properties such as porosity and high surface area to volume ratio for bone tissue engineering.
在本研究中，采用静电纺丝法制备了由聚乙烯醇（PVA）、家蚕茧丝素蛋白（SF）提取物和银纳米颗粒（Ag NPs）组成的复合纳米纤维支架，作为骨组织工程的基质。制备的原始PVA浓度为10%（重量）。分别以PVA:SF（90:10）（体积/体积）的比例制备了含丝素蛋白和银纳米颗粒的PVA复合纳米纤维支架。通过紫外可见光谱（Uv-vis）确认了AgNPs的形成和存在。使用Image J软件通过扫描电子显微镜（SEM）测定纳米纤维的直径分布。通过傅里叶变换红外光谱（FTIR）确定化学成分。使用水接触角测定润湿性。结果表明，原始PVA10的纳米纤维平均直径为108.18 nm，PVA10/SF/Ag NPs的纳米纤维平均直径为106.62 nm，平均直径无显著变化，但其形态有变化。在PVA15/SF/Ag NPs至PVA18/SF/Ag NPs时，纳米纤维平均直径随PVA浓度增加，PVA15/SF/Ag NPs为189.12 nm，PVA18/SF/Ag NPs为224.23 nm。FTIR光谱表明存在与PVA、丝素蛋白和Ag NPs化学结构相关的特征吸收峰，这表明它们之间存在良好的相互作用，是由强分子间氢键引起的。含10%（重量）PVA的支架的接触角随丝素蛋白的加入而减小，表现出亲水性。这些成果表明PVA15/SF/Ag NPs纳米纤维作为骨组织工程可能替代品的潜力。临床相关性——这确立了一种可能的PVA/SF/Ag NPs基质，其具有骨组织工程所需的特性，如孔隙率和高表面积体积比。
